CT140 RFLP Probe: Four primers were designed from the CT140 RFLP probe: PCT140F1, PCT140F2, PCT140R1 and PCT140R2 (Table 1). All four primer combinations gave the strongest bands of 750 bp with Heinz 1706 DNA (Fig. 2). Primer pairs PCT140F1/PCT140R1 and PCT140F2/PCT140R2 gave two bands. Primer pairs PCT140F1/PCT140R2 and PCT140F2/PCT140R1 gave a single band. The primer pair PCT140F2/PCT140R1 ga...

randomly amplified polymorphic dna (rapd) markers were used to study the genetic diversity and discriminate among 17 unknown genotypes (considered potentially salt tolerant) and 16 known olive cultivars. fifteen decamer primers which produced 38 reproducible polymorphic bands in the genotypes were selected for analysis. the rapd markers resulted in 93 distinct banding patterns. based on either ...

Pulsed-field gel electrophoresis techniques were used to examine the chromosomes of Pneumocystis carinii isolated from laboratory rats and two human subjects. P. carinii organisms isolated from each of four rat colonies and from two patients each produced a distinct band pattern, but in all cases the bands ranged in size from 300 to 700 kilobase pairs. P. carinii from three rat colonies produce...

FIG. 1.-Ultraviolet pH 12.15 2 photographs and densitometer tracings of typical pneumococcal DNA bands in CsCl, with reference bands of dAT polymer (1) and M. lysodeikticus DNA (2). Top: pH 12.15; mid. N D die: dAT plus nativeandi heat-enatured pneumopH7 coccal DNA, pH 7; bottom: J native, pH 7. Centrifuged 21 hr at 44,770 rpm, 2 25.00C. The dAT andM. lysodetkticus bands at pH 12 are still rath...

The cultivated apple (Malus domestica) is one of the most important fruit crops cultivated in different regions of the world including Iran. For production and breeding of high quality apple, the knowledge of genetic diversity of the cultivated apples  is  necessary. Therefore we studied genetic diversity of 25 genotypes by using ISSR molecular markers. In present study, ten ISSR primers produc...

Prunus spinosa, blackthorn, exists as wild populations that inhabit uncultivated uplands of Coruh Valley in the northeastern part of Turkey; the fruit is used to make preserves. We examined genetic diversity in wild-grown Prunus spinosa; 16 individual plants from wild populations of Coruh Valley were sampled and subjected to RAPD (random amplified polymorphic DNA) analysis. We tested 51 random ...

This study evaluated quantitatively and qualitatively the effect of the storage time of samples before the application of the cell lysis solution (CLS) for extracting DNA from buccal cells (BC). BC from the upper and lower gutter region were collected from 5 volunteers using special cytobrushes (Gentra), totaling 3 collections for each individual. In the control group (n=10), CLS was applied so...

Haematococcus pluvialis (Flotow) is a unicellular green alga, which is considered to be the best astaxanthin-producing organism. Molecular markers are suitable tools for the purpose of finding out genetic variations in organisms; however there have been no studies conducted on ISSR or RAPD molecular markers for this organism. The DNA of 10 different strains of H. pluvialis (four strains from Ir...

Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) and inter simple sequence repeats (ISSRs) markers were performed to detect the genetic diversity among 6 new rice lines and 4 cultivars with different responses to drought tolerance and establish specific DNA markers associated with drought tolerance. Among 16 RAPD primers tested, only 5 produced bands polymorphic between lin...