The present review summarizes the results of all published papers on whole-body protein turnover in man measured by [15N]glycine and the end-product method using both urea and ammonia. It begins with a short account of the underlying assumptions and the justification for the use of [15N]glycine. The results are then compared with those of a large sample of measurements by the 'gold standard' pr...

A database of peptide chemical shifts, computed at the density functional level, has been used to develop an algorithm for prediction of 15N and 13C shifts in proteins from their structure; the method is incorporated into a program called SHIFTS (version 4.0). The database was built from the calculated chemical shift patterns of 1335 peptides whose backbone torsion angles are limited to areas o...

The metabolism of the broad-spectrum herbicide, glyphosate (N-phosphonomethylglycine) in a soil Pseudomonas sp. PG2982 has been determined by cross-polarization magic-angle spinning 15N and 13C NMR of intact lyophilized cells. Using samples grown on 13C- and 15N-labeled glyphosate, we find that PG2982 does not metabolize glyphosate to aminomethylphosphonate as has been reported for mixed cultur...

Cyclic and non-cyclic bis(am ino)phosphenium cations (1 to 4) together w ith their precursors (bis(am ino)phosphorus chlorides 5 to 8) and som e other bis(am ino)phosphorus com pounds (9 to 14) have been studied by l3C, 15N (natural abundance), 29Si and 3IP N M R spectroscopy. 15N chem ical shifts and coupling constants ' / ( 31P 15N ) o f phosphenium cations have been deter­ m ined for the fir...

Recent natural catastrophes with large-scale loss of life have demonstrated the need for a new technique to provide information for disaster victim identification when DNA methods fail to yield the identification of an individual, or in other situations where authorities need to determine the recent geographical life history of people. The latter may be in relation to the identification of indi...

Coupling constants V (I5N 13CR) and V (15N 'H R) in 2-substituted pyridines [R = Me (1), CH = CH2 (2), C = C H (3), C (0)H (4), C (0)M e (5)] have been measured by using 7/ahn-echo extended (H EED) pulse sequences for one(ID ) and two-dimensional (2D ) l3C/'H N M R (HEEDINEPT, HEED-HETCOR). The magnitude of | 2/ ( ,5N 13Cr)| is hardly affected by the hybridiza­ tion o f 13Cr . 15N N M R spectra...