Recently, we have demonstrated by two different methods that lipoxgenases (LOXs) and 14-3-3 proteins form interactions in barley embryos [Holtman, Roberts, Oppedijk, Testerink, van Zeijl and Wang (2000) FEBS Lett. 474, 48-52]. It was shown by both co-immunoprecipitations and surface-plasmon resonance experiments that 13-LOX, but not 9-LOX, forms interactions with 14-3-3 proteins. In the present...

To elucidate involvement of tau protein in neurodegenerative processes in Alzheimer disease and related disorders, self-assembly process and degradative process of tau protein were examined. To understand the mechanisms of the aggregation, binding affinity of tau protein to 14-3-3 protein, which converts tau to a filamentous or aggregated form. was investigated employing a surface plasmon reson...

A bio-orthogonal click-chemistry procedure was developed to allow the in cell interactome profiling of scalaradial, an anti-inflammatory marine natural product. The results were validated through the application of the classical in vitro chemical proteomics and several bio-physical methods; peroxiredoxins, 14-3-3 isoforms and proteasomes were recognized as main scalaradial targets.

1Department of Immunology, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Australia; 2Puget Sound Blood Center and Division of Hematology, University of Washington, Seattle; 3Australian Centre for Blood Diseases, Monash University, Alfred Medical Research and Education Precinct, Melbourne, Australia; and 4Department of Human Immunology, Institute of Medical and Ve...

Chemically induced dimerization is an important tool in chemical biology for the analysis of protein function in cells. Here we report the use of the natural product fusicoccin (FC) to induce dimerization of 14-3-3-fused target proteins with proteins tagged to the C terminus (CT) of the H(+)-ATPase PMA2. To prevent nonproductive or detrimental interactions of the 14-3-3 proteins and CT fusions ...

Blue light (BL) activates the plasma membrane H(+)-ATPase via phosphorylation of the C-terminus with concomitant binding of 14-3-3 protein to the terminus in stomatal guard cells. However, the binding site and role of 14-3-3 protein in this physiological response have not been elucidated. We investigated the above using synthetic phosphopeptides designed from the C-terminus of Vicia H(+)-ATPase...

Laboratory of Plant Pathology, Faculty of Agri culture, Nagoya University , Anjo, Japan I. タ バ コ葉diskで のuracil-2-14Cの と りこ み 実験 方 法: タバ コ (品種Bright Yellow種) の切 取 葉 を中肋 か ら2分 し, 半 葉 にTMVを 接 種 , 半 葉 は 対 照 とした. 各 半 葉 か ら径12mmのdiskを 抜 き, そ れ ぞれ 別 の ペ ト リ 皿内 の 蒸 溜 水 上 に浮 か べ , これ を25°C の 恒温 箱 に お さめ, 螢 光 灯 で 連 続 照 明 した. 各 ペ トリ皿 内 のdisk数 は28枚 , 蒸 溜 水 の 液 量 は15mlで あ る. 接 種 して か ら一 定 時 間毎 に, 接 種 対 照 両 ...

PURPOSE 14-3-3 is a highly conserved, ubiquitously expressed family of proteins. At least seven mammalian isoforms (beta, epsilon, gamma, eta, theta, sigma, and zeta) are known. These proteins associate with over 200 different target molecules and activate several downstream signaling cascades involved in the regulation of metabolism, cell cycle, apoptosis, protein trafficking, transcription, s...