selected genotypes of sugar beet are often maintained by conventional propagation, but much faster rates of multiplication can be now obtained through micropropagation. on the other hand, plant micropropagation using bioreactors can decrease the cost of production and makes it an economical method. in this research, the possibility of using bioreactor and optimization of the tissue culture proc...

Clerodendrum pholomidis is an important medicinal plant of Lamiaceae. Leaves, internode and petiole explants were cultured on Murashige and Skoog (MS) medium supplemented with combinations of 2, 4-dichlorophenoxy acetic acid (2, 4-D), kinetin (kn) and indole-3 butyric acid (IBA). Leaf explants formed clusters of globular somatic embryos directly from epidermal cells within 12-15d when cultured ...

Background: Plumbagin is as an important bioactive secondary metabolite found in the roots of Plumbago spp. The only one species, Plumbago europaea L., grows wild in Iran. The therapeutic use of plumbagin is limited due to its insufficient supply from the natural sources as the plants grow slowly and take several years to produce quality roots. O...

Objective: Here, we established the protocol for plant regeneration of Psoralea corylifolia via In-vitro micro propagation. Methods: The Apical meristems was used as the explants cultured on Murashige and Skoog (1962) medium (MS) supplemented different concentrations and combinations of plant growth regulators, 6-Benzylaminopurine (BAP), Kinetin (Kn), 1-Naphthaleneacetic acid (NAA) and B5 vitam...

Background: Lemon verbena (Lippia citriodora H.B.K.) is an aromatic and medicinal plant of family Verbenaceae, which cultivated in the North region of Iran. Objective: This comparative study was carried out on essential oil content and composition of Lippia citriodora H.B.K. from micro-propagated and rooted apical stem cutting plantlets. Methods: The micro-propagated plantlets were obtained ...

An efficient protocol for in vitro shoot multiplication of Randia dumetorum (Emetic nut) has been developed. The seeds of R. dumetorum were germinated in vitro in MS medium in 5 weeks. Subsequent propagation using shoot tip as an explant was carried out in MS medium along with different concentrations and combinations of BAP (0.5-2.0) and NAA (0.0-2.0). Maximum shoot multiplication was obtained...

Atalantia monophylla (L.) DC. is a medium sized medicinal tree used in Ayurveda, Siddha and Traditional systems of medicine. It is used for the treatment of chronic rheumatism and paralysis, as an antidote for snake bites and shows antibacterial, antifungal and antifeedant activity. The study aims to develop a protocol for multiple shoot induction from nodal and root explants. After surface ste...

An in vitro plant regeneration protocol was successfully established in Aphyllorchis Montana , a saprophytic achlorophyllous orchid by culturing immature seeds. Among the six basal media evaluated for seed germination, BM-TM medium was found to be the best followed by KC medium. After 40 days, all the media turned brown and the growths of the protocorms were arrested. Activated charcoal, 1 g/l ...

An efficient procedure for shoot regeneration was obtained by endosperm culture in Actinidia deliciosa cv. Hayward. Mature endosperm cultured on MS medium supplemented with 2 mg/l 2,4-D and 5 mg/l kinetin developed callus with 80% efficiency. Callus was transferred on MS medium containing different plant growth regulators (2,4-D, TDZ, IAA, NAA, BAP, kinetin, 2iP) for regeneration. There were si...