The amyloid β peptide (Aβ) is a key player in the etiology of Alzheimer disease (AD), yet a systematic investigation of its molecular interactions has not been reported. Here we identified by quantitative mass spectrometry proteins in human brain extract that bind to oligomeric Aβ1-42 (oAβ1-42) and/or monomeric Aβ1-42 (mAβ1-42) baits. Remarkably, the cyclic neuroendocrine peptide somatostatin-1...

OBJECTIVES The objectives were to evaluate the safety, reactogenicity, and allele-specific immunogenicity of the blood-stage malaria vaccine FMP1/AS02A in adults exposed to seasonal malaria and the impact of natural infection on vaccine-induced antibody levels. DESIGN We conducted a randomized, double-blind, controlled phase I clinical trial. SETTING Bandiagara, Mali, West Africa, is a rura...

Viral vectored vaccines have been shown to induce both T cell and antibody responses in animals and humans. However, the induction of even higher level T cell responses may be crucial in achieving vaccine efficacy against difficult disease targets, especially in humans. Here we investigate the oligomerization domain of the α-chain of C4b-binding protein (C4 bp) as a candidate T cell "molecular ...

Strategies to optimize formulations of multisubunit malaria vaccines require a basic knowledge of underlying protective immune mechanisms induced by each vaccine component. In the present study, we evaluated the contribution of antibody-mediated and cell-mediated immune mechanisms to the protection induced by immunization with two blood-stage malaria vaccine candidate antigens, apical membrane ...

The present study was carryout to review the antigens of three (3) medically important Schistosomes namely: Schistosoma mansoni, S. haematobium, and japonicum. parasites-host relationship, antigens, species that produce functions were discussed. Identified include: Sm-24 kDa, Sm108 Polymorphic mucins, 28/30 kDa Protease, 47 protease, 60 Cercarial Elastase (CE) also called Cathepsin, 22.6 23 16 ...

In adrenocortical tumors, malignancy is strongly associated with insulin-like growth factor II (IGF-II) gene overexpression and abnormalities at the 11p15 locus, suggesting a role for this growth factor in adrenocortical tumorigenesis. To further investigate this role, the IGF/IGF-binding protein (IGFBP) system was analyzed in 18 adrenocortical tumors, classified into 2 groups on the basis of t...

Penelitian ini bertujuan untuk mengisolasi protein alergen belut segar (BS) dan rebus (BR) dengan buffer Tris Glisin (TG), DTT (TGD) Phosphat saline (PBS) serta mengkarakterisasi alergenisitas isolat uji elektroforesis SDS-PAGE, ELISA immunoblotting . Rendemen yang dihasilkan pada BSTG, BSTGD BSPBS sebesar 2166 mg/L, 2083 mg/L 2284 mg/L. ekstrak BRTG, BRTGD, BRPBS adalah 1445 1805 1207 Hasil SD...

 The Indian riverine buffaloes are more susceptible to variations in the environment. These variations affect seminal plasma proteins, which in turn affect fertility. This study was conducted to evaluate variations in the Indian Bubalus bubalis seminal plasma protein profile using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) during winter and summer seasons, and its relation wit...

Fasciola hepatica, the aetiological agent of fascioliasis in the Caribbean region, occurs throughout the major islands of the Greater Antilles and in localised zones on two islands (Martinique and Saint Lucia) of the Lesser Antilles. However, apart from Puerto Rico, information regarding human fascioliasis in islands of the Caribbean is out of date or unavailable, or even nonexistent as in Hait...

More than 130 known mutations in the presenilin-1 (PS1) gene result in familial Alzheimer's disease (FAD) with a mutation specific age of disease onset. These mutations increase amyloid beta 42 (A beta42) levels, and this increase has been validated in recent years as one pathogenic factor in FAD. However, further malfunctions of mutant presenilin-1 are discussed as well. In order to assess the...