Traditionally, studies on EDMD have focused on genetic changes affecting molecules involved in the development of pathology. However, emerging findings indicate that a single genetic mutation can be accompanied by a range of different phenotypes, suggesting a contribution of the epigenetic background to the disease progression. This is in line with recent works showing that changes in chromatin...

The enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) histone methyltransferase is the catalytic subunit of polycomb repressive complex 2 (PRC2), which is important for epigenetic regulation. EZH2 is highly expressed in various types of tumors, and its high-level expression promotes the progression and invasion of certain tumors. However, the expression level of EZH2 and its func...

Polycomb Repressive Complex (PRC) 2 catalyzes the H3K27me3 modification that warrants inheritance of a repressive chromatin structure during cell division, thereby assuring stable target gene repression in differentiated cells. It is still under investigation how H3K27me3 is passed on from maternal to filial strands during DNA replication; however, cell division can reinforce H3K27me3 coverage ...

Drosophila Heat Shock System as a General Model to Investigate Transcriptional Regulation M.J. Guertin, S.J. Petesch, K.L. Zobek, I.M. Min, and J.T. Lis 1 Chromatin in the Nuclear Landscape D.B. Beck, R. Bonasio, S. Kaneko, G. Li, G. Li, R. Margueron, H. Oda, K. Sarma, R.J. Sims III, J. Son, P. Trojer, and D. Reinberg 11 Cell Signaling and Transcriptional Regulation via Histone Phosphorylation ...

The Polycomb group (PcG) proteins form chromatin-modifying complexes that are essential for embryonic development and stem cell renewal and are commonly deregulated in cancer. Here, we identify their target genes using genome-wide location analysis in human embryonic fibroblasts. We find that Polycomb-Repressive Complex 1 (PRC1), PRC2, and tri-methylated histone H3K27 co-occupy >1000 silenced g...

The CTCF protein is a highly conserved zinc finger protein that is implicated in many aspects of gene regulation and nuclear organization. Its functions include the ability to act as a repressor of genes, including the c-myc oncogene. In this paper, we show that the CTCF protein can be posttranslationally modified by the small ubiquitin-like protein SUMO. CTCF is SUMOylated both in vivo and in ...

The mammalian Polycomblike protein PHF1 was previously shown to interact with the Polycomb group (PcG) protein Ezh2, a histone methyltransferase whose activity is pivotal in sustaining gene repression during development and in adulthood. As Ezh2 is active only when part of the Polycomb Repressive Complexes (PRC2-PRC4), we examined the functional role of its interaction with PHF1. Chromatin immu...