Evidence had shown the detrimental effect of prostaglandin (PG) E2 in diabetic nephropathy (DN) of STZ-induced type-1 diabetes but its role in the development of DN of type-2 diabetes remains uncertain. The present study was undertaken to investigate the regulation of PGE2 synthetic pathway and the interaction between peroxisome proliferator-activated receptor (PPAR) γ and PGE2 synthesis in the...

Effects of ethanol treatment on Leydig cell NADPH-generating enzymes and lipid profiles were studied. Ethanol treatment (3.0 g/kg. b.wt.) twice daily as a 25% (v/v) aqueous solution given to adult Wistar rats reduced the body weight, testis weight and relative weights of the seminal vesicles and ventral prostate. Serum LH and testosterone were also decreased. Similarly, the NADPH-generating enz...

PURPOSE To investigate associations between enzyme activity of glutathione reductase (GR) with and without added flavin adenine dinucleotide (FAD), glutathione peroxidase (GPX), and 6-phosphogluconic dehydrogenase (6PGDH) in the lens epithelium collected at surgery, and some nutritional and biochemical variables determined in the same individuals during the Italian-American Case-Control Study o...

AK N = 10 N = 7 N = 39 N = 5 2 0 1 1 0 3 1 0 0 1 GAPDH N = 10 N = 7 N = 34 N = 5 1 1 0 0 0 2 0 0 0 0.800 3 0 1 0 0.200 4 0 0 1 0 GDH N = 10 N = 6 N = 29 N = 5 1 0 1 0.759 0 2 1 0 0.241 1 LDH2 N = 9 N = 7 N = 45 N = 5 2 0 0.429 0.022 0 5 0 0.571 0.200 1 6 0 0 0.000 0 7 0.833 0 0.567 0 8 0.167 0 0.211 0 PEP N = 10 N = 6 N = 45 N = 4 4 0 0.417 0.000 0 5 0.250 0 0.311 0 6 0 0 0.011 0 7 0 0.583 0.13...

Ascidians, especially those belonging to the Ascidiidae, are known to accumulate extremely high levels of vanadium in vanadocytes, one type of blood (coelomic) cell. Vanadium, which exists in the +5 oxidation state in seawater, is accumulated in the vanadocytes and reduced to the +3 oxidation state. We have been trying to characterize all of the polypeptides specific to vanadocytes and to speci...

Recent progress is reviewed in the diagnostic enzymology of renal dis­ eases. Enzyme determinations in blood serum are of little value. Activity increases during hemodialysis are most likely due to fluid redistribution. Enzymes are transported from the interstitium via the lymph into the intravasal space where they accumulate. Determination in the urine of enzymes of the brushborder of the prox...