An efficient expression system in Escherichia coli for several biologically active insulin-like growth factor-I (IGF-I) fusion peptide analogues is described. These novel IGF-I fusion protein analogues have properties that make them very useful reagents in the investigation of IGF-I action. The analogues comprise an IGF-I sequence and the first 11 amino acids of methionyl porcine growth hormone...

Conflicting data on the role of metabolic syndrome (MS) in development hepatocellular carcinoma (HCC) require studies influence molecular factors that are important HCC MS, which was goal our review. Publications (scientific articles and reviews) over past 10 years were studied analyzed using databases Web Science, Scopus, PubMed, RSCI. The terms used for search “metabolic non-alcoholic fatty l...

The effect of human growth hormone (GH) and insulin-like growth factor-l (IGF-l), IGF-ll, and insulin on human plasma cell responses was studied. GH enhanced lg production and thymidine uptake in the human plasma cell lines, IM-9 and AF-10. IGF-l, but not IGF-ll or insulin, also enhanced lg production and proliferation in them. However, enhancement by GH was not mediated by IGF-I, because enhan...

In a prior report we presented evidence that insulinlike growth factor-I (IGF-I) can act in an autocrine fashion by demonstrating that FRTL-5 cells transfected with hIGF-IA fusion genes express and secrete biologically active IGF-I that renders the stimulation of DNA synthesis in FRTL-5 cells independent of heir requirement for exogenous IGFs or insulin. To determine if IGF-1’s autocrine action...

Muscle is an important target tissue for insulin-like growth factor (IGF) action. The presence of specific, high affinity IGF receptors, as well as the expression of IGF peptides and binding proteins by muscle suggest that a significant component of IGF action in this tissue is mediated through autocrine and/or paracrine mechanisms. To explore autocrine/paracrine action of IGFs in muscle, we st...

We have cloned and characterized cDNAs encoding the zebrafish IGF ligands and receptors. Sequence comparison showed that the primary structures of zebrafish IGF-I, IGF-II, and IGF-I receptors (IGF-IRs) have been highly conserved in vertebrates. In contrast to the presence of a single IGF-IR gene in mammals, two distinct IGF-IR genes, termed igf-1ra and igf-1rb, were found in zebrafish. Structur...

Components of the insulin-like growth factor (IGF) system were investigated in chondrocytes isolated from the avian growth plate. The genes for IGF-I, IGF-II, type 1 IGF receptor (IGF-R), IGF binding protein-2 (IGFBP-2), IGFBP-3, IGFBP-5 and IGFBP-7 were found to be expressed in both proliferative and hypertrophic chondrocytes. The expression of IGF-II in proliferative chondrocytes was extremel...

Pulmonary fibrosis (PF) associated with systemic sclerosis (SSc) results in significant morbidity and mortality. We previously reported that insulin-like growth factor-II (IGF-II) is overexpressed lung tissues fibroblasts from SSc patients, IGF-II fosters by upregulating collagen type I, fibronectin, TGFβ. now show augments mRNA levels of profibrotic signaling molecules TGFβ2 (p ≤ 0.01) TGFβ3 0...