background: tuberculosis (tb) is a major cause of death worldwide. finding an effective vaccine against tb is the best way to control it. several vaccines against this disease have been developed but none are completely protective. the aim of this study was to design and construct a cloning vector containing the mycobacterium tuberculosis (m. tuberculosis) heat shock protein x (hspx). methods: ...

persian gazelle (gazelle subgutturosa) is one of the most important species in the world with its wide distribution in iran the greatest number of this species in iran is associated with the gazelles in sohrain plain in sorkhabad protected region in zanjan province. considering the significance of this species in the region and the fact that no genetic studies have been conducted to determine t...

many researchers consider the use of different probes for hybridization assays as suitable for studying the genetic diversity of nitrogen fixing bacteria. in this study for asessing genetic diversity among bradyrhizobium japonicum isolates, two different probes (suca and topa) chosen from the chromosomal genome of bradyrhizobium strain usda 110 were designed, evaluated by dnaman software and im...

objective: in this study, the possibility of prenatal diagnosis of down syndrome with real-time pcr method was evaluated. in this context, optimization of a suitable method for purification of high quality dna from amniotic fluid samples was also considered. materials and methods: pregnant women who had the high risk of having babies with down syndrome were selected according to the biochemica...

objective: in this project, our aim was to construct a novel expressing vector harboring a new sequence from overlapping region of ns3 gene of hcv from infected iranian patient. materials and methods: the partial ns3 (pns3) gene was amplified by nested-rt-pcr method using sera of hcv infected patients harboring genotype 1a. after purification and cloning the pns3 into ta-cloning vector, the be...

objective: influenza virus a (h1n1) is an important subtype of the influenza respiratory viruses, which has important worldwide implications. hemagglutinin (ha), an important viral antigen, is responsible for binding to human cell receptors leading to an onset of the disease process. considering the critical role of viral attachment, this study focuses on the extraction and cloning of ha and it...

development of potent new anti-shigella agents for rapid and specific detection and treatment is of great importance. aptamers, nucleic acid oligomers capable of specific binding to a wide range of non-nucleic acid targets, may be of value for this purpose. in the present study, we used a systematic evolution of ligands by exponential enrichment (selex) process to select dna aptamers that b...