The gene for iso-1-cytochrome c from Saccharomyces cerevisiae was recloned into a pSP65 vector containing an active bacteriophage SP promoter. The iso-1-cytochrome c gene was cloned as an 856-base pair XhoI-HindIII fragment. When the resulting plasmid was digested at the HindIII site 279 bases downstream from the termination codon of the gene and transcribed in vitro using SP6 RNA polymerase, f...

To the Editor: Application of DNA or RNA probes to microbiology and virology requires probes free of vector sequences, particularly vectors of bacterial origin, such as pBR322. Incomplete purification of inserts from vector sequences can produce a falsely positive result when nucleic acid probes are applied to tissue or body fluids that may be containmated with bacteria. A plasrnid vector such ...

This study aims to explore and identify the types of Lichenes that exist at Mutis Mount Area, Noepesu Village, West Miomaffo District, Biology Education Laboratory, University Timor. research was conducted for 6 months starting from May-October 2022. is descriptive qualitative research. Sampling used a purposive sampling method. The collected were identified based on their characteristics using...