The ability of RNA to catalyze ligation is critical its central role in many prebiotic model scenarios, particular the copying information during self-replication. Prebiotically plausible ribozymes formed from short oligonucleotides can reversible cleavage and reactions, but harsh conditions or unusual scenarios are often required promote folding drive reaction equilibrium towards ligation. Her...

Abstract Recently, studies about RNA modification dynamics in human RNAs are among the most controversially discussed. As a main reason, we identified unavailability of technique which allows investigation temporal processing transcripts. Here, present nucleic acid isotope labeling coupled mass spectrometry (NAIL-MS) for efficient, monoisotopic stable both and DNA standard cell culture. We desi...

Abstract N-Acyl imidazoles are unique electrophiles that exhibit moderate reactivity, relatively long-half life, and high solubility in water. Thanks to their tunable reactivity chemical selectivity, the application of N-acyl imidazole derivatives has launched a number biology researches, which include synthesis peptide/protein, labeling native proteins interest (POIs), structural analysis func...

Extracellular vesicles (EVs) are important players in the communication between different kinds of cells by delivering their content, consisting types RNA, proteins, bioactive lipids, or signaling nucleotides, into target cells. Several EVs distinguished: (1) exosomes with sizes from 30 to 150 nm originate endosomal pathway and form intracellular multivesicular bodies (MVBs), which fuse plasma ...

Cytokine storms, oxidative stress, and hyperglycemia can enhance the risk of type 2 diabetes (T2D). Moreover, T2D may change functional structural heart. However, some signaling pathways, such as insulin resistance, dyslipidemia, hyperglycemia, play in T2D, various pathomechanics pathophysiology involved are not understood. it is well documented that non-coding RNAs potentially pivotal m...

RNA circularization made by head-to-tail back-splicing events is involved in the regulation of gene expression from transcriptional to post-translational levels. By exploiting RNA-Seq data and down-stream analysis, we shed light on the importance of circular RNAs in plants. The results introduce circular RNAs as novel interactors in the regulation of gene expression in plants and imply the comp...