The Faces Pain Scale (FPS; Bieri et al., Pain 41 (1990) 139) is a self-report measure used to assess the intensity of children's pain. Three studies were carried out to revise the original scale and validate the adapted version. In the ®rst phase, the FPS was revised from its original seven faces to six, while maintaining its desirable psychometric properties, in order to make it compatible in ...

This critical review provides an overview of the continually expanding family of fluorescent proteins (FPs) that have become essential tools for studies of cell biology and physiology. Here, we describe the characteristics of the genetically encoded fluorescent markers that now span the visible spectrum from deep blue to deep red. We identify some of the novel FPs that have unusual characterist...

Chromophore-binding domains from plant and bacterial photoreceptor proteins have recently gathered increasing attention as new sources of genetically encoded fluorescent proteins (FPs). In particular, FPs based on the flavin-binding LOV (light, oxygen, or voltage sensing) domain offer advantages over green fluorescent protein (GFP) owing to their smaller size, pH and thermal stability, utility ...

Förster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution. Fluorescent proteins (FPs) are most commonly used as both donor and acceptor fluorophores in FRET biosensors, especially since FPs are genetically encodable and live-cell compati...

In nonlinear robust identification context, a process model is represented by a nominal model and possible deviations. With parametric models this process model can be expressed as the so-called Feasible Parameter Set (FPS), which derives from the minimization of identification error specific norms. In this work, several norms are used simultaneously to obtain the FPS. This fact improves the mo...

The paper presents an architecture for fast mode decision algorithm in H.264/AVC for 4x4 intra-prediction. This algorithm is based on the inherent symmetry existing in the spatial prediction modes. This algorithm gives a faster way of calculating sum of absolute differences (SADs) for all modes of intra prediction. So, a significant computational savings can be achieved. Synthesis results confi...

87 Progress in fluorescence imaging is enabling the study of biological events at unprecedented detail, thanks to novel microscopy techniques that provide imaging with nanoscale resolution. 3D imaging of living cells within intact tissues, organs, and whole animals, is accessible through multiphoton excitation. The remarkably rich photophysics of Fluorescent Proteins (FPs) makes them flexible e...

In this paper we present work to combine fixed priority scheduling with off-line schedule construction.It assumes a schedule has been constructed off-line for a set of tasks to meet their complex constraints.Our method takes the schedule and assigns the FPS attributes priority, offset, and period, to the tasks, such that their runtime FPS execution matches the off-line schedule.It does so by di...

Computational protein annotation methods occasionally introduce errors. False-positive (FP) errors are annotations that are mistakenly associated with a protein. Such false annotations introduce errors that may spread into databases through similarity with other proteins. We present a protein-clustering method that enables automatic separation of FP from true-positive hits. The method is based ...

We show that fluorescence lifetime is a powerful contrast mechanism that can enhance the whole-body imaging of fluorescent proteins (FPs), in the presence of background tissue autofluorescence (AF). The nonexponential AF decay is characterized from time-domain (TD) measurements on multiple nude mice and separated from the FP fluorescence using a linear fit to a priori basis functions. We illust...