Histone deacetylases such as human HDAC1 and yeast RPD3 are trichostatin A (TSA)-sensitive enzymes that are members of large, multiprotein complexes. These contain specialized subunits that help target the catalytic protein to histones at the appropriate DNA regulatory element, where the enzyme represses transcription. To date, no deacetylase catalytic subunits have been shown to have intrinsic...

Although it has been known for a long time that ubiquitylation has a major role in the activation and regulation of the nuclear factor kappa B (NF-κB) pathway, recent studies have revealed that the picture is a lot more complex than originally thought. NF-κB and ubiquitylation initially became linked when it was recognised that lysine (K)48-linked ubiquitin chains are involved in the processing...

The Harary-Hill conjecture states that for every n > 0 the complete graph on n vertices Kn, the minimum number of crossings over all its possible drawings equals H(n) := 1 4 ⌊n 2 ⌋⌊n− 1 2 ⌋⌊n− 2 2 ⌋⌊n− 3 2 ⌋ . So far, the lower bound of the conjecture could only be verified for arbitrary drawings of Kn with n ≤ 12. In recent years, progress has been made in verifying the conjecture for certain ...

In the policy debate, intellectual property is often justified by what seems to be a straightforward argument: if innovators are not protected against others appropriating their ideas, incentives for innovation are suboptimally low. Now in most industries for most potential users, appropriating a foreign innovation is itself an investment decision fraught with cost and risk. Nonetheless standar...

We have performed molecular dynamics simulations of the phosphorylation domain (PD) of the regulatory light chain (RLC) of smooth muscle myosin, to gain insight into the thermodynamic principles governing the phosphorylation-induced disorder-to-order transition. Simulations were performed in explicit water under near-physiological conditions, starting with an ideal alpha-helix. In the absence o...

p73, a homolog of the tumor suppressor p53, transactivates many p53 target genes, leading to apoptosis or cellcycle arrest. p73 has recently been reported to play an important role in tumor suppression in amousemodel. Here, we show that Pirh2 physically interacted with p73 and downregulated p73 function through its E3 ligase activity. Pirh2 promoted p73 ubiquitination in vivo and in vitro. Intr...

Post-translational modifications of histones play crucial roles in the genetic and epigenetic regulation of gene expression from chromatin. Studies in mammals and yeast have found conserved modifications at some residues of histones as well as non-conserved modifications at some other sites. Although plants have been excellent systems to study epigenetic regulation, and histone modifications ar...

Direct determination of the irreversible turbulent flux of salinity in the ocean has not been possible because of the complexity of measuring salinity on the smallest scales over which it mixes. Presented is an analysis of turbulent salinity microstructure from measurements using a combined fast-conductivity/temperature probe on a slowly falling vertical microstructure profiler. Four hundred pa...

The protein p73, a homologue of the tumor suppressor protein p53, is capable of inducing apoptosis and cell cycle arrest. MDM2 is transcriptionally activated by p73 and represses the functions of p73, including p73-dependent transactivation and growth suppression. However, the molecular mechanism of this repression is unknown. In this study, we show that MDM2 mediates p73 ubiquitination. MDM2 m...