The Gly decarboxylase multienzyme complex (Gly cleavage system, EC 2.1.2.10) catalyzes the conversion of Gly into COz, NH3, and 5,10-methylene-tetrahydrofolate, reducing NAD+ to NADH. The enzyme has been isolated from animals, plants, and bacteria-in animals and plants it occurs exclusively in the mitochondrial matrix, and in photosynthetic tissue it can be found in very large amounts (Bourguig...

Potato (solanum tuberosum L. cv Katahdin) tuber discs treated with arachidonic acid become necrotic and accumulate sesquiterpenoid phytoalexins. The arachidonic acid also causes increases in both phenylalanine ammonia lyase and lignin, but no change in total alcohol-soluble phenols. Linoleic acid does not alter any of these parameters. A high concentration of nonanoic acid promotes both necrosi...

The effects of arachidonic and linoleic acids, separately and in co-operation with N-acetylglucosamine oligomers, on Solanum tuberosum plant suspension cell cultures were investigated in terms of the fluorescent oxygen-activated-species-sensitive dye 2',7'-dichlorofluorescin diacetate. The inductors used triggered extremely rapid (within 2-10 min) generation of H(2)O(2) in the cells; the majori...

In plant mitochondria, as in most other genetic systems, several enzymatic processing and modification steps are required to yield mature tRNAs from primary transcripts. Three of the enzymes involved, RNase P, 3'-processing activity, and tRNA nucleotidyl transferase, were identified in potato (Solanum tuberosum) mitochondria and have been separated by several purification steps. RNase P was par...

Chalcone synthase (CHS) gene was amplified by PCR from cDNA of Solanum tuberosum leaf tissue. The gene was cloned into pGEMT easy vector and sequenced. It was then ligated into pET 30 vector to construct recombinant plasmid pET30-CHS. The recombinant was transformed into E.coli BL21 strain and the target gene was over expressed using IPTG. Optimum condition of IPTG concentration and temperature...

Farhat Ali Khan and Shabana Irum Department of Botany, Hazara University Mansehra, Pakistan Shazia Erum and Aish Muhammad Plant Genetic Resource Institute, National Agricultural Research Centre, Islamabad, Pakistan Faisal Nouroz Department of Botany, Hazara University Mansehra, Pakistan and Molecular Genetics Laboratory, Department of Biology, University of Leicester, UK Saima Kanwal Food Scien...

Microsomal membranes from potato tubers were treated with a phospholipase C extracted from Bacillus cereus. A positive correlation could be observed between the hydrolysis of membranous phospholipids and the decrease of the NADH-cytochrome c reductase activity. Addition of total lipid or phospholipid micelles to phospholipase C-treated microsomes partially restored the NADH-cytochrome c reducta...

We have applied Metabolic Control Analysis (MCA) in an attempt to determine the distribution of glycolytic flux control between the steps of glycolysis in aged disks of potato tuber under aerobic conditions, using concentrations of glycolytic metabolites in tuber tissue from a range of transgenic potato plants and published enzyme kinetic data. We modelled the substrate and effector kinetics of...