• Massive metabolic reprogramming was unveiled for Haematoccocus reddish cells. The differential expression of 100s genes induces this reprogramming. bHLH transcription factor family is a putative astaxanthin biosynthesis regulator. Vegetative palmelloid cells are model synthesis studies. Astaxanthin valuable and highly demanded ketocarotenoid pigment, which the chlorophycean microalga Haematoc...

The main objective of this study was to evaluate the stability of astaxanthin after drying and storage at different conditions during a 9-week period. Recovery of astaxanthin was evaluated by extracting pigments from the dried powders and analysing extracts by HPLC. The powders obtained were stored under different conditions of temperature and oxygen level and the effects on the degradation of ...

Astaxanthin, a powerful antioxidant, is a good candidate for the prevention of intracellular oxidative stress. The aim of the study was to compare the antioxidant activity of astaxanthin present in two natural extracts from Haematococcus pluvialis, a microalgae strain, with that of synthetic astaxanthin. Natural extracts were obtained either by solvent or supercritical extraction methods. UV, H...

Fish that are colored in nature often acquire faded coloration under intensive culture conditions. Experiments adding top-coated algae to the diets of ornamental fish have resulted in color enhancement. Freshwater red velvet swordtails Xiphophorus helleri, rainbowfish Pseudomugil furcatus, and topaz cichlids Cichlasoma myrnae became significantly more intensely colored when fed a diet containin...

During periods of heavy exercise training and competition, lipid, protein, and nucleic molecules can become damaged due to an overproduction of reactive oxygen and nitrogen species (RONS) within the exercising organism. As antioxidants can prevent and delay cellular oxidative damage through removing, deactivating, and preventing the formation of RONS, supplementation with exogenous antioxidant ...

Cyclization of acyclic lycopene by cyclases marks an important regulatory point in carotenoid biosynthesis. Though some algal lycopene epsilon cyclases (LCYEs) have been predicted computationally, very few have been functionally identified. Little is known about the regulation mechanisms of algal LCYEs. Recent comparative genomic analysis suggested that Haematococcus pluvialis contained only th...

The reaction kinetics for the hydrolysis of astaxanthin esters and the degradation of astaxanthin during saponification of the pigment extract from the microalga Haematococcus pluvialis were investigated. Different concentrations of sodium hydroxide in methanol were used for the saponification under nitrogen in darkness at ambient temperature (22 degrees C) followed by the analysis of astaxanth...

13 14 In this work, extraction and characterization of compounds with antioxidant and antimicrobial 15 activity from Haematococcus pluvialis microalga in red phase have been carried out. To do 16 this, subcritical water extraction (SWE) has been combined with analytical techniques such as 17 HPLC-DAD, HPLC-QqQMS and GC-MS and in-vitro assays (i.e., for antioxidant and 18 antimicrobial activity)...

The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high-value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and cell deterioration or death under stress conditions (e.g., high light, nitroge...