An Anacystis nidulans photolyase enzyme containing two chromophore cofactors was simulated for a photoreaction DNA repairing process via molecular dynamics (MD) method. A novel approach has been introduced for the electron transfer between the FAD (flavin adenine dinucleotide; flavin) molecule and CPD (cyclobutane pyrimidine dimer). This approach involves four simulation stages with different c...

The cephalic phase of digestion (CPD) has been extensively investigated in terms of digestion and metabolism. Nevertheless, microcirculatory changes required to prepare peripheral tissues in order to dispose nutrients have never been assessed. In this study, microvascular function has been evaluated to determine its behavior and potential association to hormonal secretions during CPD. Thirty-ni...

Escherichia coli DNA photolyase is a DNA-repair enzyme that repairs cyclobutane pyrimidine dimers (CPDs) that are formed on DNA upon exposure of cells to ultraviolet light. The light-driven electron-transfer mechanism by which photolyase catalyzes the CPD monomerization after the enzyme-substrate complex has formed has been studied extensively. However, much less is understood about how photoly...

Recent studies indicate that renal sympathetic nerve activity is chronically suppressed during ANG II hypertension. To determine whether cardiopulmonary reflexes and/or arterial baroreflexes mediate this chronic renal sympathoinhibition, experiments were conducted in conscious dogs subjected to unilateral renal denervation and surgical division of the urinary bladder into hemibladders to allow ...

BACKGROUND Cardiac tamponade is associated with fatal outcomes for patients with acute type A aortic dissection, and the presence of cardiac tamponade should prompt urgent aortic repair. However, treatment of the patient with critical cardiac tamponade who cannot survive until surgery remains unclear. We analyzed our experience of controlled pericardial drainage (CPD) managing critical cardiac ...

The growth in professional learning is opening up many new opportunities for the application of e-learning to the world of continuing professional development (CPD). CPD programmes require professionals to engage in annual programmes of professional development, and in some cases revalidation or recertification. To date, most of this activity has been off-line. Increasingly, CPD requirements wi...

We have studied the antitumor activity of a folate-cleaving bacterial enzyme, carboxypeptidà se G, (CPD G,), which hydrolyzes the C-terminal glutamate residue from folie acid analogs. CPD GÌcompletely inhibited the growth of all murine leukemic cell lines tested in tissue culture at enzyme levels of 0.02 to 0.025 unit/ml. A decrease in cell number was apparent after 12 hr of exposure to the en...