bakground: granulocyte colony-stimulating factor (g-csf) is a cytokine that stimulates hematopoiesis and induces proliferation and differentiation of granulocyte progenitor cells as well as production of bone marrow neutrophilic granulocyte colonies.  nowadays, human recombinant g-csf(hr g-csf) is used for the treatment of chemotherapy- and radiotherapy-induced neutropenia, and also in patients...

Introduction Genetic background has known to be associated with the outcome of human T cell lymphotropic virus (HTLV) type I infection. In The present study we investigate the association between GM-CSF gene polymorphisms with the outcome of HTLV-I infection. Materials and Methods We analyzed 3 single-nucleotide polymorphisms in the promter region of granulocyte macrophage colony stimulating...

A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression inducers such as Isopropyl β-D-1-thiogalactopyranoside (IPTG) at proper time. In the present stu...

Neutrophils contribute to the pathophysiology of hidradenitis suppurativa (HS), a chronic debilitating inflammatory skin disease, yet their exact role remains be fully defined. Granulocyte colony-stimulating factor (G-CSF), major regulator neutrophil development and survival, can blocked by novel, human anti-G-CSF receptor (G-CSFR) monoclonal antibody CSL324. We investigated activation migratio...

Introduction Germinal Matrix-Intraventricular Haemorrhage (GM-IVH) is one of the most common neurological complications in preterm infants, which can lead to accumulation cerebrospinal fluid (CSF) and a major cause severe neurodevelopmental impairment infants. However, pathophysiological mechanisms triggered by GM-IVH are poorly understood. Analyzing CSF that accumulates following IVH may allow...