Heparin-binding EGF-like growth factor (HB-EGF) is a smooth muscle cell mitogen composed of both EGF receptor and heparin-binding domains. To better understand the function of its domains, intact HB-EGF or its heparin-binding (HB) domain (amino acids 1-45) were fused to a mutant Pseudomonas exotoxin with an inactivated cell-binding domain. The resulting chimeric toxins, HB-EGF-PE* and HB-PE*, w...

Hemoglobin (Hb) is the major protein in erythrocytes and carries oxygen (O2) throughout the body. Recently, Hb has been found synthesized in atypical sites, including the brain. Hb is highly expressed in A9 dopaminergic (DA) neurons of the substantia nigra (SN), whose selective degeneration leads to Parkinson's disease (PD). Here we show that Hb confers DA cells' susceptibility to 1-methyl-4-ph...

Hb E-Saskatoon and Hb G-Coushatta are rare hemoglobin variants that are not a health problem. Herein we present a Turkish woman that was diagnosed as homozygous Hb E-Saskatoon (only the second such case reported from Turkey) and a Turkish boy diagnosed as heterozygote Hb E-Saskatoon. Additionally, 2 Turkish sisters diagnosed as heterozygote Hb G-Coushatta are presented.

Hemoglobins (Hb) Yakima and Kempsey were purified from patients' blood with diethylaminoethyl cellulose column chromatography. The oxygen equilibrium curves of the two hemoglobins and the effects of organic phosphates on the function were investigated. In 0.1 M phosphate buffer, Hill's constants n for Hb Yakima and Hb Kempsey were 1.0 to 1.1 at the pH range for 6.5 to 8.0 and the oxygen affinit...

Hemoglobin Crete, 1 29 (H7) ala -k pro, is #{149} new mutant hemoglobin (Hb) with high oxygen affinity that was discovered in a Greek family in various combinations with #{176}and #{244} ’-thaIassemia. The propositus. who presented an unusual clinical picture of an “overcompenseted” hemolytic state. with erythrocytosis. splenomegaly. abnormal red cell morphology, and marked erythroid hyperplasi...

Erythrocytes were fractionated, by centrifugation, on the basis of cell density, which is proportional to cell age. Glycated hemoglobin (Hb A1c) in the fractionated cells was measured and compared with the Hb A1c in whole blood and with the fructosamine concentrations in plasma of normal and diabetic subjects. In normal subjects, Hb A1c increased according to the age of the erythrocytes, but hy...

blood specimens from 22 insulin dependent diabetic patient and 17 nontliabetic subject were collected im edta- tubes. quantitation of hb-al was performed by columne chromatography. the glycosylated hemoglobin component is increased in diabetic patient and found a significant correlation between hb-al concentration and blood glucose levels in all diabetic patients. the mean hb-al valve for diabe...

Hb F, some of which is glycated (2), accounts for the majority (70%–95%) of Hb species in newborns. About 5%– 30% is Hb A, with none to trace amounts of Hb A1c. This patient’s sample was collected after transfusion of 2 aliquots of red blood cells from a single donor. The donor’s blood contained 12.6% Hb A1c. Concentrated glucose in blood storage media increases donor Hb A1c only minimally (3, ...

Give a brief description of Haemoglobin (Hb):  Hb is made up of Four Subunits (Tetramer) held together by multiple non-covalent interactions  Each subunit (Monomer) consist of: Haem (Ferro-Protoporphyrin) and Globin protein  Haem consist of a Protoporphyrin IX and Ferrous ion (Fe 2 )  Globin protein folds around the Haem group forming a hydrophobic pocket that protects the Haem, which serve...