background and objectives: pneumonia with acinetobacter baumannii has a major therapeutic problem in health care settings. decision to initiate correct antibiotic therapy requires rapid identification and quantification of organism. the aim of this study was to develop a rapid and sensitive method for direct detection of a. baumannii from respiratory specimens. materials and methods : a taqman ...

background and objectives : streptococcus pneumoniae is the main causative agent of acute bacterial meningitis in the world. this study aimed to detect pneumoniae infection in cerebrospinal fluid (csf) samples of patients with suspected meningitis. materials and methods : in this study, 200 csf samples in patients with suspected meningitis and with negative bacterial cultures were tested. demog...

background: the effect of curcumin as a natural safe compound with different biological activities was examined on fungal growth and aflatoxin production in aspergillus parasiticus nrrl 2999. methods: the fungus was cultured in presence of serial two-fold concentrations of curcumin (125-2000 µg/ml) in yeast extract sucrose broth for 3 days at 28°c. mycelia dry weight was determined as an index ...

background: the breast cancer is the most prevalent cancer among women, on the other hand absence of myoepithelial cells play a pivotal role in pathogenesis of this cancer. thus we aimed to investigate the possible abilities of the molecular assay technique to find a relationship between mammary serine protease inhibitor (maspin) gene expression possibly secreted by myoepithelial cells, grade o...

Those who have worked in the field of quantitative polymerase chain reaction (PCR) since the early 1990s have accepted many of the tedious aspects of the early assays as routine. Difficulties associated with early quantitative PCR techniques included: (i) ensuring that the PCR was within the linear range of amplification (that portion where the PCR signal is directly proportional to the input c...

The image on this cover is of an OpenArray ® plate which is primarily used for mid-density real-time PCR on the QuantStudio™ 12K Flex system. The figure above shows the commonly used formats for real-time PCR.

The real-time polymerase chain reaction (PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the DNA amplification and detection steps into one homogeneous assay and obviates the need for gel electrophoresis to detect amplification products. Appropriate data analysis and/or use of apposite chemistries ...