BACKGROUND Conventional acid-fast bacilli (AFB) staining cannot differentiate viable from dead cells. Propidium monoazide (PMA) is a photoreactive DNA-binding dye that inhibits PCR amplification by DNA modification. We evaluated whether PMA real-time PCR is suitable for the early detection of viable Mycobacterium tuberculosis (MTB) in clinical respiratory specimens. METHODS A total of 15 dilu...

The effect of the tumor-promoting agent phorbol 12-myristate 13-acetate (PMA) on insulin receptors and insulin action was studied in rat hepatoma cells in culture. PMA (0.1-1.0 micrograms/ml) did not affect insulin binding either acutely or chronically but inhibited insulin stimulation of glycogen synthase and tyrosine aminotransferase. PMA (1 microgram/ml) stimulated the phosphorylation of the...

Monocarboxylate transporter (MCT1) plays an important role in the absorption of short-chain fatty acids (SCFA) such as butyrate in the human colon. Previous studies from our laboratory have demonstrated that phorbol ester, PMA (1 microM, 24 h), upregulates butyrate transport and MCT1 protein expression in human intestinal Caco-2 cells. However, the molecular mechanisms involved in the transcrip...

Activation of protein kinase C (PKC) regulates the activity of a number of neurotransmitter transporters. When Xenopus oocytes expressing the cloned human dopamine transporter (hDAT) were pretreated with bath-applied phorbol 12-myristate 13-acetate (PMA), a PKC activator, [3H]DA uptake decreased irreversibly in a time- and dose-dependent manner (IC50 = 22 nM; maximal inhibition = 63-85%). The i...

We determined whether resveratrol, a phenolic antioxidant found in grapes and other food products, inhibited phorbol ester (PMA)-mediated induction of COX-2 in human mammary and oral epithelial cells. Treatment of cells with PMA induces COX-2 and causes a marked increase in the production of prostaglandin E2. These effects were inhibited by resveratrol. Resveratrol suppressed PMA-mediated incre...

Ribosomal protein S6 kinase activity was measured in lysates prepared from serum-deprived chicken embryo fibroblasts (CEF) treated for various times with phorbol 12-myristate 13-acetate (PMA). Maximal activity was observed within 15 min, and it declined to the initial level by 4 hr. Incubation of these cells with PMA 4-60 hr after the initial treatment did not result in an additional increase i...

Phorbolol myristate acetate (PHMA) had been previ ously prepared from the potent mouse skin tumor pro moter phorbol myristate acetate (PMA) by sodium borohydride reduction of the C-5 carbonyl group in PMA to a secondary alcohol. PHMA was shown to have an inflam matory effect in mouse skin equal to that of PMA. 2,3Dihydrophorbol myristate acetate (DPMA), a new com pound, was prepared from the 3-...

Flexible magnetic materials with robust and controllable perpendicular anisotropy (PMA) are highly desirable for developing flexible high-performance spintronic devices. However, it is still challenge to fabricate PMA films through current techniques of direct deposition on polymers. Here, we report a facile method synthesizing single-crystal freestanding SrRuO3 (SRO) membranes controlled cryst...

This paper aims to evaluate the performance of synchronous reluctance motors assisted by a permanent magnet (PMa-SynRM) focused on efficiency and torque pulsations. PMa-SynRM shows high power factor, compared induction (IM), although they have greater cost. These machines develop relatively ripple, cogging torque, imbalances. Consequently, electromagnetic is reduced, motor temperature increased...

The development of graphene oxide/rare earth complexes luminescent materials is limited due to the strong quenching ability oxide. However, hybrid with pyromellitic acid (PMA) as ligand not only have better performance, but also stronger luminescence intensity. Herein we tested effect carboxylic structure PMA on intensity materials. Phthalic (PA), Isophthalic (i-PA) and Terephthalic (p-PA) are ...