Proper disposal of As-hyperaccumulator Pteris vittata biomass (Chinese brake fern) enhances its application in phytoremediation. The goal of this study was to optimize As removal from P. vittata (PV) biomass by testing different particle sizes, extractants, extraction times and solid-to-liquid ratios. PV biomass was extracted using different extractants followed by different Mg-salts to recover...

fractions of bracken fern-fed cattle and of cows bearing spontaneous urinary bladder tumors contained some carcinogenic substance(s) which induced tumors in the bladder of the calf, dog, rat, or mouse and tumors in the skin of mice. It is not known whether this carcinogenic activity is attributable to bracken fern itself or to its metabolites. It has been shown (22) that the carcinogenicity of ...

Arsenic (As)-resistant bacteria are abundant in the rhizosphere and tissues of As-hyperaccumulator Pteris vittata. However, little is known about their roles in As transformation and As uptake in P. vittata. In this study, the impacts of P. vittata tissue extracts with or without surface sterilization on As transformation in solutions containing 100 μg L-1 AsIII or AsV were investigated. After ...

In the rat hippocampal formation, degeneration of CA4-derived afferent fibers provokes the growth of mossy fiber collaterals into the fascia dentata. These aberrant fibers subsequently form granule cell-granule cell synapses. The hippocampal slice preparation was employed to determine whether these recurrent connections are electrophysiologically functional. Hippocampal slices were prepared 12 ...

Results of neural grafting to excitotoxic and ischemic lesions of the adult rat hippocampus is reviewed with particular emphasis on the exchange of host-transplant nerve connections. Based on observations obtained by a variety of tracing techniques about 6 weeks after--(i) grafting of pieces of neonatal fascia dentata to one week old ibotenic acid lesions of the adult rat fascia dentata, (ii) g...

The activity of choline acetyltransferase (ChAT), acetylcholinesterase (AChE), and muscarinic receptors was studied in control rats and in rats growth-retarded in utero because of reduction of the blood supply 5 days before birth. The different markers of the cholinergic system were estimated at P (postnatal day) 6, 9, 12, 15, 22 and 60 in cerebellum, hypothalamus, septum, striatum and CA1, CA3...

Neurons in the dentate hilus or area CA3c of rat hippocampal slices were recorded intracellularly with electrodes containing the fluorescent dye Lucifer yellow. Stimulation of perforant path fibers in the molecular layer of the fascia dentata strongly excited most hilar neurons, with a much lower threshold for action potential generation than granule cells and area CA3c pyramidal cells that wer...

Recently a commissural fiber projection that terminates in the outer molecular layer of the fascia dentata was described in normal rats (Deller et al., 1995). In the present article, Phaseolus vulgaris leucoagglutinin (PHAL) tracing was used to analyze the contribution of this previously unknown projection to the commissural sprouting response after entorhinal cortex lesion. Rats 4-8 weeks afte...

To investigate the changes underlying kindling epileptogenesis in the rat hippocampus, the levels of the messenger RNAs encoding for the subunits of the N-methyl-D-aspartate-receptor (1, 2A-D) and the kainate-receptor (1, -2, GluR-5, -6, -7) were determined in hippocampal principal neurons using in situ hybridization techniques and semi-quantitative analysis of the autoradiograms. Schaffer coll...

We studied the binding of [35S]t-butylbicyclophosphorothionate to the GABAA receptor-mediated chloride channel in the CA1 area and fascia dentata of control and Schaffer collateral kindled rats, by means of semi-quantitative autoradiography. The [35S]t-butylbicyclophosphorothionate binding was determined at three stages during kindling acquisition: (i) after six afterdischarges, (ii) after 14 a...