Twenty-nine Korean wheat cultivars were analyzed for 8 important minerals (Cu, Fe, Mn, Zn, Ca, K, Mg and P) using Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES). A hierarchical cluster analysis (HCA) was applied to classify wheat cultivars, which has a similarity in mineral compositions. The concentration ranges of the micro-minerals Cu, Fe, Mn, and Zn: 0.12~0.71 mg/100 g, 2....

ZnO and Mn-ZnO nano powders were prepared by the sol-gel auto combustion method. The products were characterized by X-ray diffraction (XRD), energy dispersive analysis of X-ray (EDX) and scanning electron microscopy (SEM). Structural and morphological properties of nano particles were investigated and the average crystalline size of ZnO and Mn-doped ZnO was obtained 44 and 51 nm, respectively. ...

The kinetic properties of the purified pyruvate kinase (ATP: pyruvate phosphotransferase, EC 2.7.1.40) from the spore-forming bacterium Bacillus licheniformis were investigated. Catalysis by this enzyme in vitro required both monovalent and divalent cations and proceeded optimally at pH 7.0 to 7.4. The activity of purified pyruvate kinase was modulated by substrate activation (both P-enolpyruva...

ZnO and Mn-ZnO nano powders were prepared by the sol-gel auto combustion method. The products were characterized by X-ray diffraction (XRD), energy dispersive analysis of X-ray (EDX) and scanning electron microscopy (SEM). Structural and morphological properties of nano particles were investigated and the average crystalline size of ZnO and Mn-doped ZnO was obtained 44 and 51 nm, respectively. ...

RNase E is a major intracellular endoribonuclease in many bacteria and participates in most aspects of RNA processing and degradation. RNase E requires a divalent metal ion for its activity. We show that only Mg(2+) and Mn(2+) will support significant rates of activity in vitro against natural RNAs, with Mn(2+) being preferred. Both Mg(2+) and Mn(2+) also support cleavage of an oligonucleotide ...

Class I cyclic nucleotide phosphodiesterases (PDEs) share a catalytic domain containing 18 invariant residues. In cGMP-binding cGMP-specific PDE (PDE5), we showed previously that point mutation of nine of these profoundly decreases k(cat) when the assay is conducted in the presence of Mg(2+); seven of these are in the prototypical metal-binding motifs A and B (HX(3)HX(n)()E) that we identified ...

Iron (Fe) and copper (Cu) metabolism are closely intertwined, and a deficiency of Cu may lead to a secondary Fe deficiency. The impact of a severe Cu deficiency on proteins involved in Fe metabolism in the bovine has not been studied. Therefore, a 493 day study was conducted to determine the effect of a severe long-term Cu deficiency on Fe metabolism in beef cattle. Twenty-one Angus calves were...