نتایج جستجو برای: zona free oocyte

تعداد نتایج: 537619  

Journal: :Annales de Biologie Animale Biochimie Biophysique 1977

2016
Ayuko UCHIKURA Hitomi MATSUNARI Kazuaki NAKANO Shota HATAE Hiroshi NAGASHIMA

A novel hollow fiber vitrification (HFV) method was applied to materials that have previously been difficult to cryopreserve, thereby expanding the potential application of this method. The results showed that zona-free porcine morulae and their isolated blastomeres remained viable even after vitrification. The rate of development to blastocysts after vitrification was similar for zona-free and...

2012
Bartosz Kempisty Marta Jackowska Magdalena Woźna Paweł Antosik Hanna Piotrowska Piotr Zawierucha Dorota Bukowska Jędrzej M. Jaśkowski Michał Nowicki Klaus P. Brüssow

Cumulus-oocyte-complexes (COCs) were collected from small (<3 mm), medium (3-5 mm), and large (>5 mm) porcine follicles, and the INHA and INHB expression and cellular localization were studied. Developmentally competent (BCB+) COCs were cultured for 44 h. Samples of mRNA were isolated before and after in vitro maturation (IVM) from oocytes collected from follicles of different size for RQ-PCR a...

Seyed Mohammad Ali Shariatzadeh & Zahra Alizadeh Department of Biology, Faculty of Sciences, Arak University, Arak, Iran Correspondent author: Seyed Mohammad Ali Shariatzadeh, [email protected] Abstract. Silver nanoparticles (Ag NPs) can affect female fertility because they can cause toxicity in the ovaries. The aim of this study is to see if Spirulina platensis (SP) can protect m...

Journal: :Development 2001
X Shi S Amindari K Paruchuru D Skalla H Burkin B D Shur D J Miller

ZP3 is a protein in the mammalian egg coat (zona pellucida) that binds sperm and stimulates acrosomal exocytosis, enabling sperm to penetrate the zona pellucida. The nature of the ZP3 receptor/s on sperm is a matter of considerable debate, but most evidence suggests that ZP3 binds to beta-1,4-galactosyltransferase-I (GalTase) on the sperm surface. It has been suggested that ZP3 induces the acro...

Journal: :journal of reproduction and infertility 0

background: sperm vitrification is a technique of ice and cryoprotectant free cryopreservation by direct plunging of sperm suspension into liquid nitrogen (ln2). the aim of this study was to investigate the influence of cryoprotectant free-vitrification on human sperm fine structure by msome technology and the fertility potential by zona binding assay (zba). methods: 20 normo-ejaculates were pr...

Journal: :Biology of Reproduction 1976

Asgharnia M Bahadori MH Faraji R Ghasemian F, Zahiri Z

Background: Abnormal oocyte morphology has been associated with the hormonal environment to which the gametes are exposed. In this study, we evaluated the oocytes morphology, fertilization rate, embryos quality, and implantation rate resulted of retrieved oocytes in different times after human chorionic gonadotrophin (hCG) administration. Materials and Methods: A total of 985 metaphase II were ...

Journal: :iranian journal of basic medical sciences 0
hamid reza asgari department of anatomy, faculty of medicine, tehran university of medical sciences, tehran, iran mohammad akbari department of anatomy, faculty of medicine, tehran university of medical sciences, tehran, iran mehdi abbasi department of anatomy, faculty of medicine, tehran university of medical sciences, tehran, iran jafar ai department of tissue engineering, school of advanced technologies, tehran university of medical sciences, tehran, iran morteza korouji department of anatomy, faculty of medicine, iran university of medical sciences, tehran, iran fereshte aliakbari department of anatomy, faculty of medicine, tehran university of medical sciences, tehran, iran

objective(s): the present day challenge is how to obtain germ cells from stem cells to treat patients with cancer and infertility. much more efforts have been made to develop a procedure for attaining germ cells in vitro. recently, human umbilical cord-derived mesenchymal stem cells (humscs) have been introduced with higher efficacy for differentiation. in this work, we tried to explore the eff...

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