In several recent studies, proteomics analyses suggest that increase of ubiquinol-cytochrome c reductase core protein 1 (UQCRC1) is cardio-protective. However, direct evidence for this effect has not yet been obtained. Thus, the current study aimed to determine this effect and the mechanism underlying this effect. The results showed that overexpression of UQCRC1 protected H9c2 cardiac cells aga...

ASPND1 and ASPF2 are immunodominant antigens from Aspergillus nidulans and A. fumigatus, respectively, that are readily synthesized in infections in the human host, as demonstrated by their reactivity with more than 80% of sera from patients with aspergilloma or allergic bronchopulmonary aspergillosis. We demonstrate here that both antigens are exclusively produced under situations of low bioav...

A novel polyfunctional dipodal ligand, L = N,N'-bis[2-[(2-hydroxy-1-naphthyl)methyleneamino]ethyl]propanediamide (DOTA2HNAP) was developed and characterized through elemental and spectral analyses. The complexation behavior of the ligand was investigated with Co2+, Cu2+, and Zn2+ metal ions by potentiometric and spectrophotometric methods in H2O-D...

Properties of solid phases formed during the oxidation of EDTA by permanganate in a high-pH, high-ionic strength solution, and the retention of Cu2+, Ni2+, and Zn2+ by them were examined. Morphologically, the solids were agglomerates of particles with sizes <100 nm. X-ray absorption spectroscopy (XAS) analysis indicated that these particles were birnessite. Its precipitation was accompanied by ...

Recent studies have demonstrated an important role for T-type Ca2+ channels (T-channels) in controlling the excitability of peripheral pain-sensing neurons (nociceptors). However, the molecular mechanisms underlying the functions of T-channels in nociceptors are poorly understood. Here, we demonstrate that reducing agents as well as endogenous metal chelators sensitize C-type dorsal root gangli...

The dephosphorylation of ATP was characterized by determining the dependence of the firstorder rate constant on pH in the absence and presence of Zn2+ and together with Zn2+ and 2,2'-bipyridyl. The Zn2+-accelerated reaction passes through a pH optimum at about 8. The decrease in the rate at higher pH is due to the formation of Zn(ATP) (OH)3-; this species is relatively in­ sensitive towards dep...

PURPOSE To examine the effects of Zn2+ on glutamate-induced neurotoxicity in cultured retinal neurons. METHODS Primary cultures obtained from fetal rat retinas (16 to 19 days gestation) were used. The neurotoxic effects of excitatory amino acids were quantitatively assessed using the trypan blue exclusion method. RESULTS A brief exposure of retinal cultures to glutamate or N-methyl-D-aspart...

Zinc ion fluorescence sensing and the binding properties of 4-methyl-2,6-bis(((phenylmethyl)imino)methyl)phenol (HL) have been investigated. It displays high selectivity for Zn2+ and can be used as zinc ion-selective luminescent probe for biological application under physiological conditions. The increase in emission in the presence of Zn2+ is accounted for by the formation of hexanuclear compl...

The bacterial heat shock protein Hsp33 is a redox-regulated chaperone activated by oxidative stress. In response to oxidation, four cysteines within a Zn2+ binding C-terminal domain form two disulfide bonds with concomitant release of the metal. This leads to the formation of the biologically active Hsp33 dimer. The crystal structure of the N-terminal domain of the E. coli protein has been repo...

In this work, we studied nonlinear dynamics and optical limiting (OL) effects of pulse trains in zinc porphyrins meso-tetrakis methylpyridiniumyl (Zn2+TMPyP) sulfonatophenyl (Zn2+TPPS) porphyrin J-aggregates. The environments were selected as aqueous solutions micelles sodium dodecyl sulfate (SDS) cetyltrimethyl ammonium bromide (CTAB). Our numerical results show that both Zn2+TMPyP Zn2+TPPS ar...