BACKGROUND Recombinant protein production is universally employed as a solution to obtain the milligram to gram quantities of a given protein required for applications as diverse as structural genomics and biopharmaceutical manufacture. Yeast is a well-established recombinant host cell for these purposes. In this study we wanted to investigate whether our respiratory Saccharomyces cerevisiae st...

BACKGROUND Microbial bioconversion of photosynthetic biomass is a promising approach to the generation of biofuels and other bioproducts. However, rapid, high-yield, and simple processes are essential for successful applications. Here, biomass from the rapidly growing photosynthetic marine cyanobacterium Synechococcus sp. PCC 7002 was fermented using yeast into bioethanol. RESULTS The cyanoba...

In order to exploit a fast-growing Paulownia hardwood as an energy crop, xylose-enriched hydrolysate was obtained in this work increase the ethanol concentration using hemicellulosic fraction, besides already widely studied cellulosic fraction. For that, elongata x fortunei submitted autohydrolysis treatment (210 °C or S0 of 4.08) for xylan solubilization, mainly xylooligosaccharides. Afterward...

Over the last few decades, efforts to transition global production of fuels and chemicals toward renewable carbon feedstocks have accelerated. A large portion these focused on valorization one most abundant sources, lignocellulose. Pretreatment lignocellulose is first critical step in this process. In study, novel ionic liquid (IL) systems consisting multiple ions known be effective at biomass ...

A sequencing batch reactor (SBR) inoculated with activated sludge and bioaugmented a dye-decolorizing yeast strain—Yarrowia lipolytica (HOMOGST27AB) was assembled to form yeast-bioaugmented aerobic granular (AGS). The AGS-SBR operated for the treatment of synthetic saline wastewater (12 g L−1) intermittently fed reactive textile dye (Navy Everzol ED) at 25, 15, 7.5 mg L−1. Dye degradation did n...

Exopolysaccharides (EPS) of fungal origin have attracted special attention from researchers due to their multifarious applications in the food and pharmaceutical industries. In the present study, optimization of the process parameters for the production of exopolysaccharide by Schizophyllum commune AGMJ-1 was studied using one factor at a time (OFAT) method, Plackett-Burman design (PBD) and res...

Although an increasing number of horizontal gene transfers have been reported in eukaryotes, experimental evidence for their adaptive value is lacking. Here, we report the recent transfer of a 158-kb genomic region between Torulaspora microellipsoides and Saccharomyces cerevisiae wine yeasts or closely related strains. This genomic region has undergone several rearrangements in S. cerevisiae st...