Calcium (Ca(2+))-mediated signaling is a conserved mechanism in eukaryotes, including the human malaria parasite, Plasmodium falciparum. Due to its small size (<10 μm) measurement of intracellular Ca(2+) in Plasmodium is technically challenging, and thus Ca(2+) regulation in this human pathogen is not well understood. Here we analyze Ca(2+) homeostasis via a new approach using transgenic P. fal...

Two optimal algorithms, MTA* and MT-RBA*, are presented to find the optimal yard crane (YC) job sequence for serving a fleet of vehicles for delivery and pickup jobs with scheduled deadlines and predicted vehicle arrival times. The objective is to minimize the total tardiness of incoming vehicle jobs. This is important for minimizing vessel turnaround time. In the search for an optimal job sequ...

The late S/G2-specific transcription of the human cdc25C gene is dependent on an initiator-proximal repressor element (CDE) and an upstream activating sequence (UAS) of undefined nature. We now show that these upstream sequences harbour multiple in vivo protein binding sites that interact with transcriptional activators and form separable, context-independent functional modules. Major component...

Understanding how bacterial communities adapt to different environmental factors provides a scientific basis for developing and utilizing microbial resources in rivers. This study investigated the changes of water mud samples from two sites an urban river (GH: Gonghe Village YC: Yanchuan). Analysis showed that site GH had higher concentrations ammonium, total nitrogen, Mn, Ni than YC. High-thro...

Interleukin (IL) 2 signaling requires the dimerization of the IL-2 receptor f3 (IL-2Rf3) and common y ('yc) chains. The yc is also a component of the receptors for IL-4, IL-7, and IL-9. To assess the extent and role of the receptor signal transducing system utilizing the yc chain on human intestinal epithelial cells, the expression of yc, IL-2Rj3, and receptor chains specific for IL-4, IL-7, an...

Concepts such as participation and environment may differ across cultures. Consequently, to use a measure like the Young Children’s Participation Environment Measure (YC-PEM) in other than original English-speaking contexts, cultural adaptation needs be assured. The objective is cross-culturally translate, adapt, pilot test YC-PEM into Dutch language culture. In total 3 cognitive interviews wit...

This study examined the impact of irrelevant dimensional variation on rule-based category learning in patients with Parkinson's disease (PD), older controls (OC), and younger controls (YC). Participants were presented with 4-dimensional, binary-valued stimuli and were asked to categorize each into 1 of 2 categories. Category membership was based on the value of a single dimension. Four experime...

Scaffold molecules interact with multiple effectors to elicit specific signal transduction pathways. CIITA, a non-DNA-binding regulator of class II major histocompatibility complex (MHC) gene transcription, may serve as a transcriptional scaffold. Regulation of the class II MHC promoter by CIITA requires strict spatial-helical arrangements of the X and Y promoter elements. The X element binds R...

Histones are among the most conserved proteins in evolution, sharing a histone fold motif. A number of additional histonic proteins exist and are involved in the process of transcriptional regulation. We describe here the identification, cloning and characterization of two small members of the H2A-H2B sub-family (YBL1 and YCL1) related to the NF-YB and NF-YC subunits of the CCAAT-binding activa...