Previous studies from this laboratory (1) have demonstrated the existence of several different types of “xanthine oxidase” in nature. The enzymes in milk and mammalian tissues appear to be quite similar, but can be differentiated by the inhibitor Antabuse, which affects only the tissue enzyme (2). Antabuse can also be used to separate the dehydrogenase and oxidase activities of tissue xanthine ...

Xanthine oxidase activity containing fractions from rat, mouse, rabbit and guinea pig livers were obtained by heat treatment and ammonium sulfate precipitation. Xanthine oxidase activity was observed in rat and mouse liver fractions, while xanthine oxidase activity was absent in rabbit and guinea pig liver fractions. Enzyme kinetic parameters, K m and V max , were determined for the conversion ...

Background:  The development of nanotechnology has been a boon to mankind as its significance paved the way for several applications in therapeutics. This study was to evaluate the anti-arthritic capability of nano Selenium-lovastatin mixture (Lov-Se) against inflammatory cascade in arthritic irradiated rats. Materials and Methods: Animal model of Arthritis was organized by subcutaneous in...

Oxygen metabolites have been implicated in the pathogenesis of various types of acute tissue injury. One biologic source of oxygen metabolites is the reaction catalyzed by the enzyme xanthine oxidase. Because we previously demonstrated that the substrates for xanthine oxidase (hypoxanthine and xanthine) are elevated in the plasma of critically il l patients, we questioned whether the enzyme its...

As the enzyme responsible for uric acid formation, Xanthine oxidase was considered to be a therapeutic target hyperuricemic treatment. This study carried out assess potential of caryophyllene, and its derivates usually present in natural product inhibit oxidase. The molecular docking using Autodock Tool Biovia Discovery Studio conducted visualize interaction reveal structure-activity relationsh...

Two procyanidin fractions, namely oligomers and polymers isolated from grape seed methanolic extract were characterized. Phenolic composition and procyanidin purity of these fractions were determined by normal-phase and reverse-phase HPLC, thioacidolysis-HPLC, ESI-MS analyses, formaldehyde-HCl precipitation and elemental analysis. Antioxidant activities of these fractions and other well-known a...

Although it has been established that oxidative stress mediates cytotoxicity by familial Alzheimer's disease (FAD)-linked mutants of presenilin (PS)1 and that pertussis toxin inhibits cytotoxicity by FAD-linked N141I-PS2, it has not been determined whether oxidative stress is involved in cytotoxicity by N141I-PS2 or which pertussis toxin-sensitive proteins mediate the cytotoxicity. Here we repo...

Influenza virus infection is associated with development of oxidative stress in lung and blood plasma, viz. increase of primary and secondary lipid peroxidation products. It was established that rimantadine treatment led to a decrease of the products of lipid peroxidation in tissues of mice experimentally infected with influenza virus A/Aichi/2/68 (H3N2). The effect is strongest in blood plasma...

In 1948 Miller (1) demonstrated that the loss of liver xanthine oxidase activity in the rat greatly exceeded the loss of liver protein during acute inanition. Moreover, xanthine oxidase appeared to be the most labile of the four liver enzymes studied. Westerfeld and Richert (2) observed that increasing the level of protein in the diet tended to bring about an increase in liver xanthine oxidase ...

Previous papers examined lipid peroxidase levels and myeloperoxidase activity as products of oxidative and inflammatory reactions in the tear fluid of patients suffering from dry eye. The aim of the present paper was to investigate whether the enzymes xanthine oxidoreductase/xanthine oxidase known to generate reactive oxygen species contribute to oxidative reactions on the ocular surface. Xanth...