نتایج جستجو برای: when considering his habitus

تعداد نتایج: 1692680  

Journal: :Nucleic acids research 1999
W Pan E Ravot R Tolle R Frank R Mosbach I Türbachova H Bujard

The Plasmodium falciparum malaria parasite is the causative agent of malaria tropica. Merozoites, one of the extracellular developmental stages of this parasite, expose at their surface the merozoite surface protein-1 complex (MSP-1), which results from the proteolytic processing of a 190-200 kDa precursor. MSP-1 is highly immunogenic in humans and numerous studies suggest that this protein is ...

Journal: :Protein and peptide letters 2002
Nadia A P Nogueira Moema B Grangeiro Rodrigo M S da Cunha Marcio V Ramos Maria A O Alves Edson H Teixeira Manoel Barral-Netto Juan J Calvete Benildo S Cavada Thalles B Grangeiro

ConBr, a D-glucose/D-mannose-specific lectin from Canavalia brasiliensis seeds, was produced in Escherichia coli from a (c)DNA clone subcloned to pET15b expression vector. The recombinant lectin (rConBr) was purified by one-step immobilized metal-affinity chromatography using an amino-terminal hexahistidine tag. By SDS-PAGE and Western blot, rConBr was highly pure with an apparent molecular mas...

Journal: :Microbial Cell Factories 2008
Magnólia de A Campos Marilia S Silva Cláudio P Magalhães Simone G Ribeiro Rafael PD Sarto Eduardo A Vieira Maria F Grossi de Sá

BACKGROUND Heterologous protein expression in microorganisms may contribute to identify and demonstrate antifungal activity of novel proteins. The Solanum nigrum osmotin-like protein (SnOLP) gene encodes a member of pathogenesis-related (PR) proteins, from the PR-5 sub-group, the last comprising several proteins with different functions, including antifungal activity. Based on deduced amino aci...

Journal: :The journal of physical chemistry. C, Nanomaterials and interfaces 2016
Gerald Manuel Andrej Lupták Robert M Corn

A two-step templated, ribosomal biosynthesis/printing method for the fabrication of protein microarrays for surface plasmon resonance imaging (SPRI) measurements is demonstrated. In the first step, a sixteen component microarray of proteins is created in microwells by cell free on chip protein synthesis; each microwell contains both an in vitro transcription and translation (IVTT) solution and ...

2015
Paul K. Kienker Zhengyan Wu Alan Finkelstein

Low pH triggers the translocation domain of diphtheria toxin (T-domain), which contains 10 α helices, to insert into a planar lipid bilayer membrane, form a transmembrane channel, and translocate the attached catalytic domain across the membrane. Three T-domain helices, corresponding to TH5, TH8, and TH9 in the aqueous crystal structure, form transmembrane segments in the open-channel state; th...

Journal: :The Journal of biological chemistry 1999
Q Hang L Woods M Feiss C E Catalano

The terminase enzyme from bacteriophage lambda is composed of two viral proteins (gpA, 73.2 kDa; gpNu1, 20.4 kDa) and is responsible for packaging viral DNA into the confines of an empty procapsid. We are interested in the genetic, biochemical, and biophysical properties of DNA packaging in phage lambda and, in particular, the nucleoprotein complexes involved in these processes. These studies r...

Journal: :Applied and environmental microbiology 2006
Andrea Campisano Christine Schroeder Mirle Schemionek Joerg Overhage Bernd H A Rehm

The function of pslD, which is part of the psl operon from Pseudomonas aeruginosa, was investigated in this study. The psl operon is involved in exopolysaccharide biosynthesis and biofilm formation. An isogenic marker-free pslD deletion mutant of P. aeruginosa PAO1 which was deficient in the formation of differentiated biofilms was generated. Expression of only the pslD gene coding region resto...

Journal: :Protein expression and purification 2003
Arun K Mohanty Chad R Simmons Michael C Wiener

Affinity tags such as polyhistidine greatly facilitate recombinant protein production. The solubility of integral membrane proteins is maintained by the formation of protein-detergent complexes (PDCs), with detergent present at concentration above its critical micelle concentration (CMC). Removal of the affinity tag necessitates inclusion of an engineered protease cleavage site. A commonly util...

Journal: :Protein expression and purification 1999
C M Hill R G Duggleby

Ketol-acid reductoisomerase (EC 1.1.1.86) catalyzes the conversion of 2-aceto-2-hydroxyacids to 2-keto-3-hydroxyacids and their subsequent reduction by NADPH to 2,3-dihydroxyacids. The gene encoding the Escherichia coli enzyme was cloned and expressed as a hexahistidine-tagged fusion protein and the recombinant enzyme purified by metal-ligand affinity chromatography. The pure enzyme was tested ...

Journal: :The Journal of biological chemistry 2003
Antonio Ferreira-Pereira Sergio Marco Annabelle Decottignies Joseph Nader André Goffeau Jean-Louis Rigaud

Pdr5p, the major multidrug exporter in Saccharomyces cerevisiae, is a member of the ATP-binding cassette (ABC) superfamily. Pdr5p shares similar mechanisms of substrate recognition and transport with the human MDR1-Pgp, despite an inverted topology of transmembrane and ATP-binding domains. The hexahistidine-tagged Pdr5p multidrug transporter was highly overexpressed in yeast strains where other...

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