Two protein phosphatases (enzymes I and II) were extensively purified from wheat embryo by a procedure involving chromatography on DEAE-cellulose, phenyl-Sepharose CL-4B, DEAE-Sephacel and Ultrogel AcA 44. Preparations of enzyme I (Mr 197,000) are heterogeneous. Preparations of enzyme II (Mr 35,000) contain only one major polypeptide (Mr 17,500), which exactly co-purifies with protein phosphata...

A soluble enzyme system which catalyzes the aerobic oxidation of reduced pyridine nucleotides in wheat germ has previously been described (1). Peroxidase was shown to be a component of this system. Later, Humphreys (2) demonstrated that Mn+ and an unidentified cofactor were required for aerobic oxidation of TPNH’ in crude preparations of malic enzyme from wheat germ. Although no attempt was mad...

Wheat, rice, and corn are the most widely eaten whole grains, followed by oats, rye, and barley. A wheat kernel consists of 80–85% endosperm, 10–14% bran, and 2.5–3% germ (Figure 1). Whole grains contain a high amount of bioactive substances such as fiber, vitamins, minerals, antioxidants, and other phytochemicals such as betaine, choline, sulfur amino acids, and melatonin, which account for at...

Model experiment was conducted with growing pigs fed diets containing zero or two levels (1 or 2 g/kg) of fermented wheat germ extract (FWGE). In the first part of the experiment (19 days between 49 and 68 days of age) weight gain and feed conversion were tested. The 2nd part of the experiment (21 days between 68 and 89 days of age) served to measure the effect of FWGE on some compartments of t...

BACKGROUND Identification of wheat sensitization by a skin prick test (SPT) is essential for children with wheat-induced anaphylaxis, since oral food challenge can cause serious adverse effects. Wheat allergens are both water/salt and alcohol soluble. The preparation of wheat extract for SPT containing both water/salt and alcohol soluble allergen is needed. OBJECTIVE To determine if a wheat e...

Five experiments were conducted to determine the effects of different wheat gluten (WG) sources (Source 1 = enzymatically hydrolyzed, Source 2 = nonmodified ring-dried, Source 3 = spray-dried, and Source 4 = flash-dried) on growth performance of nursery pigs compared with soybean meal (SBM), spray-dried animal plasma (SDAP), or other specialty protein sources. In Exp. 1, pigs (n = 220, initiall...

170 broilers have been infected with Salmonella infantis and vaccinated against IBD,(infectious bursal disease) IB, (infectious bronchitis) ND. (Newcastle disease)Feed of 85 broilers has been completed with FWGE (fermented wheat germ extract) Shedding-characteristics of Salmonella infantis and immune reactions after vaccinations were investigated with both broiler groups. Intensity and dynamics...

BACKGROUND Fermented wheat germ extract (FWGE) sold under the trade name Avemar exhibits anticancer activity in vitro and in vivo. Its mechanisms of action are divided into antiproliferative and antimetabolic effects. Its influcence on cancer cell metabolism needs further investigation. One objective of this study, therefore, was to further elucidate the antimetabolic action of FWGE. The antica...

In vitro-transcribed, unmodified, and non-aminoacylated amber suppressor tRNAs that are recognized by natural aminoacyl-tRNA synthetase were improved toward higher suppression efficiency in batch-mode cell-free translation in wheat germ extract. The suppression efficiency of the suppressor obtained through four sequence optimization steps (anticodon alteration of natural tRNAs (the first genera...