Characterization of the periplasmic region of PomB, a Na + -driven flagellar 1 stator protein in Vibrio alginolyticus 2 3 4 Na Li 1,2 , Seiji Kojima 2* and Michio Homma 2 5 6 1 Division of Microbiology, Graduate School of Life Science, Northwest A&F 7 University, Yanglin, Shaanxi, Yanglin 712100, China; 2 Division of Biological 8 Science, Graduate School of Science, Nagoya University, Furo-cho,...

The bacterial flagellar motor has a stator and a rotor. The stator is composed of two membrane proteins, MotA and MotB in Escherichia coli and PomA and PomB in Vibrio alginolyticus. The Vibrio motor has a unique structure, the T ring, which is composed of MotX and MotY. Based on the structural information of PomB and MotB, we constructed three chimeric proteins between PomB and MotB, named PotB...

The biological effects of supernates obtained from different strains of Vibrio alginolyticus and Vibrio anguillarum isolated from diseased fish have been studied by inoculation on two fish species, eel and rainbow trout, and two fish cell lines. These supernates possess neuroexcitatory properties, and so, when they are injected into both fish species, they trigger convulsions, wriggling, contor...

Garlic contains various nutrients and healthful ingredients. The content of garlic products is closely related to their processing methods, each compound has specific biological effects. Allicin, a heat labile compound, considered the most important factor in garlic. powder commonly prepared by subjecting raw thermal before being grounded into powder,and it interest compare activity with fish. ...

Two hundred and eighty four presumptive but not confirmed Vibrio vulnificus isolates grown on cellobiose-polymixin B-colistin agar (CPC) at 40 degrees C, recovered from sea water samples from Valencia, Spain, during a microbiological survey for V. vulnificus, were phenotypically identified. Most of the isolates (91%) corresponded to Vibrio species. V. harveyi (24%) and V. splendidus(19%) were t...

Vibrio parahaemolyticus, a gram-negative marine bacterium, is a worldwide cause of food-borne gastroenteritis. Recent genome sequencing of the clinical V. parahaemolyticus strain RIMD2210633 identified two sets of genes for the type III secretion system (TTSS), TTSS1 and TTSS2. Here, we constructed a series of mutant strains from RIMD2210633 to determine whether the two putative TTSS apparatus ...

Vibrio alginolyticus ZJ-51 displays phase variation between opaque/rugose colonies (Op) and translucent/smooth colonies (Tr). These colony variants show great differences in biofilm formation and motility. In this study, a gene encoding for an rpoS-like sigma factor, rpoX, has been cloned and characterized. The absence of rpoX did not affect colony switching rate but did decrease biofilm format...

CRYSTAL STRUCTURE AND MUTATIONAL ANALYSIS OF AMINOACYLHISTIDINE DIPEPTIDASE FROM VIBRIO ALGINOLYTICUS REVEAL A NEW ARCHITECTURE OF M20 METALLOPEPTIDASES Chin-Yuan Chang, Yin-Cheng Hsieh, Ting-Yi Wang, Yi-Chin Chen, Yu-Kuo Wang, Ting-Wei Chiang , Yi-Ju Chen, Cheng-Hsiang Chang, Chun-Jung Chen*, and Tung-Kung Wu* Department of Biological Science and Technology, National Chiao Tung University, Hsi...

Toxicity of the extracellular products (ECP) and the lethal attribute of serine protease secreted by five pathogenic Vibrio alginolyticus strains from various sources in kuruma prawn Penaeus japonicus were studied. The ECPs of organisms originally isolated from diseased kuruma prawn or small abalone Haliotis diversicolor supertexta were more lethal (LD50 value of 0.48 or 0.41 microg protein/g p...