this study was conducted to determine polymorphism of gola-drb3 in nadushan goat using pcr-rflp. blood samples were randomly taken from 100 goats. dna was extracted from the blood samples using diatom dna prep kit. spectrophotometer and 1% agarose gel were used for determining its quantities and qualities. exon 2 of drb 3.2 gene encompassing 285 bp was amplified with heminested-pcr method in tw...

In the present study, the polymorphism of myostatin gene (MSTN) in native chickens of Western Azerbaijan Rearing and Breeding Institute was investigated. The blood samples were collected from eighty two randomly selected hens. Genomic DNA was extracted from blood samples and a fragment of myostatin including 599 bp in promoter and exon 1 was amplified using PCR method. Breeding values for body ...

Objective(s): Short tandem repeat (STR) loci are the most informative DNA genetic markers for attempting to individualize biological material for application in paternity and forensic cases. Materials and Methods: Blood samples were collected and the total genomic DNA was extracted. The DNA samples were used for genotyping VWA and TPOX STR loci using PCR and polyacrylamide gel electrophoresis. ...

BACKGROUND/AIMS Tests for whether observed genotype proportions fit Hardy Weinberg Equilibrium (HWE) are widely used in population genetics analyses, as well as to evaluate quality of genotype data. To date, all methods testing for HWE require subjects to be classified into discrete categories, yet it is becoming clear that the distribution of allele frequencies tends to be smooth over geograph...

The Asiatic topmouth gudgeon, Pseudorasbora parva, is recognized as one of the most invasive fish species in many countries outside of Asia. We isolated and characterized 19 microsatellite loci from P. parva. The polymorphism of these 19 loci was tested on 40 individuals of P. parva sampled from a wild population located in Ezhou, Hubei province of China. The loci had 5 to 11 alleles, with a me...

The use of posterior probabilities to summarize genotype uncertainty is pervasive across genotype, sequencing and imputation platforms. Prior work in many contexts has shown the utility of incorporating genotype uncertainty (posterior probabilities) in downstream statistical tests. Typical approaches to incorporating genotype uncertainty when testing Hardy-Weinberg equilibrium tend to lack cali...

Moroccan goats are characterized by the presence of different populations identified only based on their phenotypes. The objectives of this study were to assess the genetic differentiation of the Draa goat breed and to analyze its genetic structure and its relationships with other local populations using 12 microsatellite markers. The screening was done in South Eastern and Southern Morocco on ...

Four Saudi Arabian indigenous sheep populations including Najdi, Hbsi, Arb, and Naemi were genotyped for 16 microsatellite markers recommended by the food and agriculture organization (FAO). This study aims to provide information on the genetic structure of the breeds analyzed and give indications and proposals for the cross breeding program. All loci were polymorphic in all populations and loc...