BACKGROUND The use of testicular sperm in assisted reproduction depends on the availability of sperm in wet preparations. It is not always possible to recover sperm from the testis, even with previous sperm-positive histopathological findings. The purpose of this study was to evaluate the sperm-negative wet preparation search results with flow cytometric ploidy analysis and histopathological ex...

The selection of sperm cells possessing normal morphology and motility is crucial for many assisted reproductive technologies (ART), especially for intracytoplasmic sperm injection (ICSI), as sperm quality directly affects the probability of inducing healthy pregnancy. We present a novel platform for real-time quantitative analysis and selection of individual sperm cells without staining. Towar...

The expression of proteins in accessory sex gland fluid (AGF) of proven, high use mature Holstein bulls was evaluated. Thirty-seven bulls with documented fertility based on their non-return rates were studied. AGF was obtained by artificial vagina after bulls were surgically equipped with cannulae in the vasa deferentia. Samples of AGF were evaluated by two-dimensional SDS-PAGE, gels stained wi...

This paper addresses the synthesis of a novel reactionless spatial 6-DOF parallel mechanism using four-bar linkages without any counter-rotations. The mechanism is first described and its kinematics is discussed. A numerical example of the reactionless 6-DOF mechanism is given in this paper and, with the help of the dynamic simulation software ADAMS, it is shown that the mechanism is reactionle...

About ten years ago, the first serious applications of concept lattices in software analysis were published. Today, a wide range of applications of concept lattices in static and dynamic analysis of software artefacts is known. This overview summarizes important papers from the last ten years, and presents three methods in some detail: 1. methods to extract classes and modules from legacy softw...

Background: Basal level of reactive oxygen species (ROS) is essential for sperm acrosomal reaction, hyperactivation, motility, capacitation and fertilization. Ram spermatozoa contain large quantities of polyunsaturated fatty acids in their plasma membranes so are extremely susceptible to ROS induced damage. Materials and Methods: Eight rams (36 ± 5 month of age and with a weight of 64 ± 7 kg) w...

background: sperm dna fragmentation is known as an important cause of male infertility. the influence of sperm dna damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial. in this study, we investigated differences of the semen parameters and intracytoplasmic sperm injection (icsi) outcome according to sperm dna fragme...

objective: melatonin, the chief secretory product of the pineal gland, regulates dynamic physiological adaptations that occur in seasonally breeding mammals as a response to changes in daylight hours. because of the presence of melatonin in semen and the membrane melatonin receptor in spermatozoa, the impact of melatonin on the regulation of male infertility is still questionable. the aim of th...

Understanding how components interact with their neighboring components is a necessary prerequisite for the evolution of legacy software systems. Dynamic program analysis is known to provide deep insight in component interaction protocols, however such techniques must all cope with a tremendous scaleability problem. Therefore, this paper proposes a heuristic which reduces program traces based o...