Ligand binding to myoglobin in aqueous solution involves two kinetic components, one extramolecular and one intramolecular, which have been interpreted in terms of two sequential kinetic barriers. In mixed solvents and sub-zero temperatures, the outer barrier increases and the inner barrier splits into several components, giving rise to fast intramolecular recombination. The nature of these bar...

The p53 tumor-suppressor protein is a cellular phosphoprotein and a negative regulator of cell growth. Most p53 mutations occur in exons 5-8 within the DNA-binding domain. Therefore, p53 can potentially be targeted with novel drugs designed to bind to a mutation and restore its stability or wild-type conformation. For the current study, Hartree-Fock calculations were used to investigate the sol...

Tissue engineering benefits from novel materials with precisely tunable physical, chemical and mechanical properties over a broad range. Here we report a practical approach to prepare Bombyx mori silk fibroin hydrogels using the principle of non-solvent induced phase separation (NIPS). A combination of reconstituted silk fibroin (RSF) and methanol (non-solvent), with a final concentration of 2....

A perfluorinated carboxylic acid membrane, i.e. Flemion, shows improved performance as actuator material compared with Nafion (perfluorinated sulfonic acid). Flemion has a higher ion exchange capacity and good mechanical strength. In particular, Flemion will deform with no back relaxation under applied electrical stimulus. However, with water as solvent, the operation of Flemion in air has seri...

In this study, novel CuO/g-C3N4 nanocomposite was simply synthesized by impregnation of g-C3N4 with CuO nanoparticles. Then, the heterogeneous catalyst was characterized by various techniques including Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and transmission electron microscopy (...

2 3 3 3 2 Na[BH(C H N ) ]·solvent (solvent = H O, DMF, IPA) crystallize as a porous framework with the distorted primitive cubic (pcu) topology. Solvent molecules occupy the framework pores. The solvent of crystallization can be removed by heating. Isothermal thermogravimetric analysis shows that solvent loss is consistent with a 1-D diffusion mechanism. Variable temperature powder X-ray diffra...

We assess the contribution of each coordination state to the hydration free energy of a distinguished water molecule, the solute water. We define a coordination sphere, the inner-shell, and separate the hydration free energy into packing, outer-shell, and local, solute-specific (chemical) contributions. The coordination state is defined by the number of solvent water molecules within the coordi...

Most proteins are typically folded into predetermined three-dimensional structures in the aqueous cellular environment. However, proteins can be exposed to a nonpolar environment under certain conditions, such as inside the central cavity of chaperones and unfoldases during protein degradation. It remains unclear how folded proteins behave when moved from an aqueous solvent to a nonpolar one. H...

Pseudomonas sp. strain ST-200, which is capable of conversion of cholesterol, was isolated from humus soil. This organism effectively modified cholesterol dissolved in an organic solvent by dehydrogenation and oxygenation. When the organism was grown in a medium overlaid with a 10%/ volume of a mixed organic solvent (p-xylene and diphenylmethane; 3:7, vol/vol) containing cholesterol (20 mg/ml),...