during the summer of 2004, a survey was conducted to determine the incidence and distribution of beet necrotic yellow vein virus (bnyvv) and its vector (polymyxa betae keskin) in marvdasht, fars province of iran.  a total of 353 root samples were collected randomly as well as 124 samples from plants showing rhizomania- associated symptoms.  the presence of bnyvv in the samples were tested by tr...

Foot-and-mouth disease (FMD) is a trans-boundary viral disease of livestock, which causes huge economic losses and constitutes a serious infectious threat for livestock farming worldwide. Early diagnosis of FMD helps to diminish its impact by adequate outbreak management. In this study, we describe the development of a real-time reverse transcription recombinase polymerase amplification (RT-RPA...

To explore the regulation and potential mechanism of long non-coding ribonucleic acid taurine upregulated gene 1 on apoptosis cell cycle osteoblasts is main purpose. The overexpression plasmid small interfering were used to regulate expression in osteoblasts. was detected by quantitative reverse transcription polymerase chain reaction effect flow cytometry, western blot analyses detect division...

The post-entry events of HIV-1 infection occur within reverse transcription complexes derived from the viral cores entering the target cell. HIV-1 cores contain host proteins incorporated from virus-producing cells. In this report, we show that MCM5, a subunit of the hexameric minichromosome maintenance (MCM) DNA helicase complex, associates with Gag polyprotein and is incorporated into HIV-1 v...

The contractile and associated metabolic characteristics of muscles are determined by their myosin heavy chain (MHC) isoform expression. In large mammals, the level of MHCIIB expression, which is associated with fast glycolytic-type muscle fibers, has not been fully characterized. In this study, quantitative reverse transcription-PCR and SDS-PAGE methodologies were developed for the analyses of...

Buckwheat rutin has been found to be able to inhibit angiotensin II (AngII) - induced hypertrophy in cultured neonatal rat cardiomyocytes, but the mechanism remains uncertain. In this study, myocardial hypertrophy model was made by adding AngII to the medium of cardiac myocytes of neonatal rats, meanwhile, different concentrations of buckwheat rutin were applied to observe their effects. Intrac...