The enzyme kinetics of estrogen 2-hydroxylase from rat liver microsomes has been investigated using two radiotracer methods. The conversion of [4-14C]estradiol to [4-14C]2-hydroxyestradiol by microsomes was examined by isolation of the catechol estrogen. [6,7-3H]2-Hydroxyestradiol was prepared and added at the end of the incubation as a recovery marker for quantitation. Initial velocity conditi...

In order to describe the relationship between lipid dynamics and enzyme function in endoplasmic reticulum membranes of the adrenal cortex, a study of temperature dependence of the 3P-hydroxysteroid dehydrogenase activity has been carried out at three different pH values, in relation to the change of the rotational motion of the membrane lipids. At pH 8.5, corresponding to the highest value of t...

This paper presents a new approach to combine cross-correlation functions. The combination is based on a maximum-likelihood approach and uses a non-linear combination scheme. It can be effective for radial-velocity analysis of multi-order spectra, or for analysis of multiple exposures of the same object. Simulations are presented to show the potential of the suggested combination scheme. The te...

Longslit spectroscopy is entering an era of increased spatial and spectral resolution and increased sample size. Improved instruments reveal complex velocity structure that cannot be described with a one-dimensional rotation curve, yet samples are too numerous to examine each galaxy in detail. Therefore, one goal of rotation curve measurement techniques is to flag cases in which the kinematic s...

The complex interactions existing between turbulence and mixing in a bluffbody stabilised flame configuration is investigated by means of a wavelet crosscorrelation analysis on Large Eddy Simulations. The combined approach allows to better point out typical features of unsteady turbulent flows with mixing through the characterisation of the processes involved both in time and scales. The wavele...

Longslit spectroscopy is entering an era of increased spatial and spectral resolution and increased sample size. Improved instruments reveal complex velocity structure that cannot be described with a one-dimensional rotation curve, yet samples are too numerous to examine each galaxy in detail. Therefore, one goal of rotation curve measurement techniques is to ag cases in which the kinematic str...

The glide-path scans of the LIDARs at the Hong Kong International Airport (HKIA) are used to calculate the eddy dissipation rate (EDR), the internationally adopted metric for turbulence reporting/alerting, by using the structure function approach based on the radial velocity data. The EDR so determined is compared with the turbulence intensity calculated from the Quick Access Recorder (QAR) dat...

Data collected over more than eight consecutive hours between two source-receiver arrays in a shallow water environment are analyzed through the physics of the waveguide invariant. In particular, the use of vertical arrays on both the source and receiver sides provides source and receiver angles in addition to travel-times associated with a set of eigenray paths in the waveguide. From the trave...