A continuous-flow apparatus is described for automatically plotting substrate saturation curves, and is suitable for use with a variety of enzymes. A linear concentration gradient of the variable substrate is combined with a fixed proportion of the other substrates and the enzyme, and after passing through a reaction coil the product concentrations are measured spectrophotometrically. Use of a ...

Members of the SLC36 family proton-coupled amino acid transporters are involved in membrane transport acids and derivatives [29, 30]. The four show variable tissue expression patterns expressed various cell types at plasma-membrane intracellular organelles. PAT1 is luminal surface small intestine absorbs [4]. In lysosomes, functions as an efflux mechanism for produced during intralysosomal prot...

Abstract The temperature-dependent resistivity of heavily Al- and N-codoped 4H-SiC grown by physical vapor transport (PVT) with Al concentrations (C ) higher than 10 19 cm −3 is investigated to obtain high-growth-rate low-cost p + -type substrates suitable for the collectors n-channel insulated-gate bipolar transistors. compared that Al-doped chemical deposition (CVD). In band conduction region...

We demonstrate the dramatic effect of film thickness on the ferroelectric phase transition temperature Tc in strained BaTiO3 films grown on SrTiO3 substrates. Using variable-temperature ultraviolet Raman spectroscopy enables measuring Tc in films as thin as 1.6 nm, and a film thickness variation from 1.6 to 10 nm leads to Tc tuning from 70 to about 925 K. Raman data are consistent with synchrot...

Diabetes mellitus is the most common metabolic disease, and has late complications that are due to chronic hyperglycaemia. Altered carbohydrate and lipid metabolism together with impaired detoxification of carbonyl substrates and impaired trapping of oxygen radicals are responsible for cell damage in diabetes. Variable functional capacity of detoxifying systems could contribute to differing sus...

Despite a growing interest in graphene, an aspect which is less studied the electrical and optical characterization of graphene oxide (GO)-based transparent conductors obtained using thermal annealing. In addition, few research works have properties GO reduced (RGO) films impedance measurements. this study, electric measurements are performed on thermally dip-coated glass substrates. The resist...

Presently, it is difficult to measure the characteristic impedance of embroidered microstrip line for its conductive anisotropy directly. The published work had proposed use S-parameters estimate port a balanced dipole antenna, and cannot be used calculate lines with one arm. Thus, still problem on measuring obtaining 50 Ω line. Therefore, taking reflection coefficient as an intermediate variab...

this experiment was conducted at research greenhouse of college of agriculture, ferdowsi university of mashhad in 1381-1382. the experiment was designed based on the splitted plots in the form of completely randomized design and in four replications. irrigation intervals were in three levels of 12, 4, and 2 times per day at primary plots, and three substrates of new perlite, used perlit, and ri...

objective(s): microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules.   materials and methods: in this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-l-lysine (pll). some of the aldehyde groups of the activated agaro...

the impact of three different fabaceous host plants including cowpea (vigna unguiculata), chickpea (cicer arietinum), and mung bean (vigna radiata) seeds was investigated using biochemical approaches on possible changes of gut proteolytic activity of the cowpea weevil, callosobruchus maculatus at 30±1˚c and 70±5% rh and a photoperiod of 8:16 (l:d). results revealed that ph of 4-5 and 9 was opti...