A multiplex PCR (MPCR) for detection of vanA-and vanB-mediated resistance to vancomycin was optimized and adapted for use in the routine microbiology laboratory. Consecutive specimens (1196) submitted for vancomycin resistant Enterococci (VRE) surveillance were processed by clinical technologists on Bile Esculin Azide Agar containing 6 mg/L vancomycin (BEAA/Vanco6) plates and 466 showing black ...

Vancomycin is a member of the glycopeptide class of antibiotics. Vancomycin resistance (van) gene clusters are found in human pathogens such as Enterococcus faecalis, Enterococcus faecium and Staphylococcus aureus, glycopeptide-producing actinomycetes such as Amycolotopsis orientalis, Actinoplanes teichomyceticus and Streptomyces toyocaensis and the nonglycopeptide producing actinomycete Strept...

BACKGROUND Early identification of pathogens and antimicrobial resistance in bloodstream infections (BSIs) decreases morbidity and mortality, particularly in immunocompromised patients. The aim of the present study was to compare real-time polymerase chain reaction (PCR) with commercial kits for detection of 17 pathogens from blood culture (BC) and 10 antimicrobial resistance genes. METHODS A...

18 19 The aim of this study was to develop a method for detecting antibiotic resistance 20 (AR) genes in cheese based on a combination of multiplex PCR and a DNA microarray 21 hybridisation system. Twenty oligonucleotide probes were designed targeting 10 22 common AR genes, namely aac(6’)-Ie-aph(2’’)-Ia, aadE, aphA-3, ermB, tet(L), tet(M), 23 tet(O), tet(S), vanA and vanB. Specificity of the pr...

OBJECTIVE The objective of the study was to investigate the prevalence of vancomycin-resistant Staphylococcus aureus (VRSA). MATERIALS AND METHODS Three hundred and fifty-six S. aureus isolates from the Imam Khomeini hospital in Tehran, Iran, were evaluated for methicillin and decreased vancomycin susceptibility by the microbroth dilution method. The mecA, vanAand vanB genes were targeted by ...

Background: Enterococci are intrinsically resistant/tolerant to various antimicrobial agents and can also acquire combine different mechanisms of resistance, including quorum-sensing regulation, most active compounds, which makes enterococcal infection treatment even more challenging. The aim this study was evaluate the pattern resistance analyze frequency asa1 esp genes in clinical isolates re...

VanD-type resistance to glycopeptides in Enterococcus faecium BM4339 is due to constitutive synthesis of D-alanyl-D-lactate-terminating peptidoglycan precursors (B. Périchon, P. Reynolds, and P. Courvalin, Antimicrob. Agents Chemother. 41:2016-2018, 1997). The sequence of a 5,780-bp fragment was determined and revealed six open reading frames. The 3' distal part encoded the VanHD dehydrogenase,...