Three vancomycin-dependent clinical isolates of Enterococcus faecalis of the VanB type were studied by determining (i) the sequence of the ddl gene encoding the host D-Ala:D-Ala ligase and the vanSB-vanRB genes specifying the two-component regulatory system that activates transcription of the vanB operon, (ii) the level of expression of resistance genes by using DD-dipeptidase activity as a rep...

Los enterococos se han convertido en patógenos oportunistas refractarios a la farmacoterapia antimicrobiana. Con el objetivo de identificar las especies y analizar resistencia Enterococcus spp vancomicina linezolid, analizaron 721 cepas obtenidas pacientes del Hospital Especialidades “José Carrasco Arteaga”- Ecuador, entre enero 2019-diciembre 2021. La especie más frecuente fue E. faecalis (73,...

BACKGROUND Glycopeptides such as vancomycin are frequently the antibiotics of choice for the treatment of infections caused by methicillin resistant Staphylococcus aureus (MRSA). For the last 7 years incidence of vancomycin intermediate S. aureus and vancomycin resistant S. aureus (VISA and VRSA respectively) has been increasing in various parts of the world. The present study was carried out t...

Surveillance cultures for vancomycin-resistant enterococci (VRE) are time-consuming and expensive for the laboratory to perform. Therefore, we investigated the use of PCR as an alternative method of detecting and identifying VRE directly in fecal samples. PCR primers directed to vanA, vanB, vanC1, vanC2, and enterococcal ligase genes were used to detect and identify VRE in fecal material obtain...

A real-time PCR assay previously developed for use on the Roche LightCycler platform was investigated as an alternative to culture for the direct detection of vancomycin-resistant enterococci (VRE) in clinical specimens. PCR primers and fluorescence resonance energy transfer hybridization probes specific for the vanA and vanB genes were combined in a multiplex real-time PCR assay performed dire...

BACKGROUND Vancomycin-resistant enterococci (VRE) are important nosocomial pathogens and food chain has been considered as an assumed source for dissemination of VRE to human. OBJECTIVES The presence of VRE isolates from food samples and typing of these isolates with Phene plate, a biochemical fingerprinting method, were investigated. MATERIALS AND METHODS Thirty samples of meat, chicken an...

The goal of this study was to assess the presence of enterococci species presenting van-mediated glycopeptide resistance in French cattle. Fecal samples were collected from healthy and sick animals, and enterococci were screened for vancomycin resistance. Vancomycin resistance was principally encountered in Enterococcus gallinarum and Enterococcus casseliflavus strains. However, glycopeptide re...

A set of well characterized strains, collected in Polish hospitals, including Gram-negative (n = 93) and Gram-positive (n = 90) isolates was used in the study. The VITEK 2 AST-cards were used in the analysis according to the manufacturer's recommendations. Comparison of the susceptibility data obtained by the standard method and by VITEK 2 cards proved concordant in 99% of cases. Clinically imp...

The World Health Organization (WHO) has defined Vancomycin intermediate Staphylococcus aureus (VISA) and resistant (VRSA) as high priority pathogens. VISA VRSA are produced by different mechanisms, hence cannot convert to VRSA. Consequently, upon vancomycin treatment of VSSA isolates, there can be emergence but not its conversion But we observed that when (MIC ≤2 µg/mL) lacking vanA or vanB gen...