We previously reported a mouse monoclonal antibody (MAb), termed L2, specific for Helicobacter pylori urease strongly inhibited its enzymatic activity. Here, to gain insight into how this antibody affects urease activity, the epitope that was recognized by the antibody was determined. By screening a panel of overlapping synthetic peptides covering the entire sequence of the two subunits (UreA a...

Ammonia, one of the main substances livestock odor, is reported to act as a major precursor secondary fine dust along with impact odor on public health due deterioration air quality, so systematic emission management needed. Livestock production ammonia mainly generated by hydrolysis urea in urine urease, hydrolytic enzyme. Therefore, this study, we aimed suppress inhibiting activity urease usi...

BACKGROUND Pathogen-specific metabolic pathways may be detected by breath tests based on introduction of stable isotopically-labeled substrates and detection of labeled products in exhaled breath using portable infrared spectrometers. METHODOLOGY/PRINCIPAL FINDINGS We tested whether mycobacterial urease activity could be utilized in such a breath test format as the basis of a novel biomarker ...

This study aimed to evaluate the effect of different nitrogen fertilizer sources and rates on morphophysiological characteristics use efficiency maize. The experiment was set in Campos Novos, Santa Catarina State, Brazil, during 2016/2017 2017/2018 growing seasons. experimental design a randomized block with treatments arranged split plots. Four were evaluated main plots: conventional urea, pro...

Urease catalyzes hydrolysis of urea. The present investigation was undertaken to investigate the urease activity and its optimization during L-glutamic acid fermentation by a biotin auxotroph Corynebacterium glutamicum X680 using urea as principal nitrogen source. Another interesting part this study efficiency microorganism for utilization indigenous raw materials (such hydrolysates cassava sta...

Density functional theory calculations were used to examine the role of the urease model complex [Ni2(bdptz)(micro-OH)(micro-H2O)(H2O)2](OTs)3(bdptz=1,4-bis(2,2'-dipyridylmethyl)-phthalazine; OTs=tosylate) in the degradation of urea. An elimination mechanism that converts urea to ammonium cyanate was investigated in detail. The lowest energy pathway involves urea coordination through the oxygen...

The measurement of blood urea nitrogen (BUN) as serum or plasma urea is one of the most frequently ordered laboratory tests for evaluating renal functions. Conventional analysis of reactions of urea with diacetyl monoxime or urease with the Berthelot reaction [1] is rapidly giving way to the totally enzymatic reactions that are more convenient to perform and more easily adapted to modern instru...

Helicobacter hepaticus is a pathogen of rodents, which causes diverse enteric and hepatic inflammatory diseases and malignancies. The urease enzyme is an important colonization factor of gastric Helicobacter species like Helicobacter pylori, but little is known about the role and regulation of urease in enterohepatic Helicobacter species. Here it is reported that urease activity of H. hepaticus...