Interest in phenolic compounds has recently increased due to their potential as antioxidant agents. Moringa leaves (Moringa oleifera) have a high content of compounds, which presents them promising source for the extraction these compounds. However, it is necessary use analytical techniques identify and quantify actual compound determine quality raw material final product. Ultrasound assisted g...

This application note describes a fast UHPLC method for the determination of 20 ginsenosides. The developed method allows for the determination of 20 ginsenosides in less than 35 minutes. A binary high pressure gradient is used at a flow rate of 0.6 ml/min. By applying a Bluespher 100-2 C18 column in the dimension 150 x 2 mm ID, the total run time could be reduced to less than 41 minutes when a...

A simple reverse-phase UHPLC (RP-UHPLC) analytical method was developed and validated according to International Council on Harmonization (ICH) guidelines for simultaneous estimation of ofloxacin (OFX) fluconazole (FCZ) using a Response surface aided central composite design (CCD, Design-Expert®, Version 11.1.2.0). The linearity, selectivity, sensitivity, accuracy, precision, limits detection q...

The aim of this study was to develop and validate a dissolution test for favipiravir release in tablet dosage form using ultra-high performance liquid chromatography (UHPLC). method developed by testing the solubility media with different pH values. results demonstrated that best achieved phosphate buffer 6.8. amount released about 100% after 30 min. UHPLC presented linearity (R = 1.000) concen...

Abstract This study aimed to investigate the use of pulsed electric fields (PEF) (2 kV/ cm; 1.5 KJ kg−1) as pre-treatment hot air drying process for improvement quality and functional properties saffron. Analysis flavouring, aroma colouring strength (E1%λmax), crocins pattern (UHPLC-MS/MS), total polyphenol content (TPC) antioxidant activity (AOA) were performed after processing during aging at...