In this study we investigated the status of jejunal absorption and peripheral metabolism of glucose following disinhibition of paraventricular nucleus (PVN) induced by the GABAA antagonist bicuculline methiodide (BMI). Adult male Wistar rats (270-300g) were anesthetized to implant unilateral guide cannula targeted to PVN for later microinjection of BMI (10 pmol/100 nl, n=6) or vehicle (NaCl 0.9...

Coronary arterial inflow is impeded and venous outflow is increased as a result of the decrease in coronary vascular volume due to cardiac contraction. We evaluated whether cardiac contraction is influenced by interfering with the changes of the coronary vascular volume over the heart cycle. Length-tension relationships were determined in Tyrode-perfused rat papillary muscle and when coronary v...

Ferritin, added to the incubation medium of ascites tumor cells, was used as an electron microscopic marker to study the uptake of large protein molecules by morphologically intact cells. A definite uptake could be detected after 1 hour of incubation in Tyrode bicarbonate solution containing 0.04 to 13.3 mg ferritin/ml. Ferritin was found in a variety of membrane-surrounded structures, suggesti...

Trypsin-dispersed cells from hearts (ventricles) of 7 to 8 day chick embryos were cultured 3 to 21 days. The cells became attached to the culture dish and assembled into monolayer communities. By means of a bridge circuit, one microelectrode was used for simultaneously passing current and recording membrane potentials (V(m)). The input resistance, calculated by the measured DeltaV(m) for a know...

We tested the hypothesis that nitric oxide (NO) produced within the carotid body is a tonic inhibitor of chemoreception and determined the contribution of neuronal and endothelial nitric oxide synthase (eNOS) isoforms to the inhibitory NO effect. Accordingly, we studied the effect of NO generated from S-nitroso-N-acetylpenicillamide (SNAP) and compared the effects of the nonselective inhibitor ...

This work deals with the effects of the alcoholic extracts of 15 plants (collected from the northern region of Pakistan) on isolated guinea-pig ileum preparation. Dried powdered plant material was extracted with alcohol. The extract obtained after the removal of the alcohol was triturated with pet-ether (40-60°) and then charcoaled, filtered and dried under vacuum. The isolated guinea-pig ileum...

The cellular effects of trypsinization, a commonly used method for separating epithelial from mesenchymal tissues, were examined with the electron microscope. Specimens were fixed after each step of the trypsinization process in 1 % OsO4 in double strength Tyrode solution and after 1-7 days of culture following reapposition of the epithelium to its own mesenchyme or collagen-rich 12-day embryon...

METHODS AJÕ the tumors1 were received as subcutaneous transplants on OSH mice. On the llth day after inoculation the animals were sacrificed, and the tumors were explanted under sterile conditions. The cultures .were explanted in Carrel flasks, roller tubes, or coverslips. The best .results were obtained with expiants cultivated in Carrel flasks. Depending on the method used there were differ ...

Electron microscopic examination of the spermatozoa from a man suffering from asthenozoospermia (poor or low sperm motility) showed that approximately 92% of the sperm flagella lacked central pair microtubules but possessed dynein arms and radial spokes while a small percentage of the spermatozoa had complete flagella. The characteristics of the motor apparatus of the spermatozoa and the effect...

The average guinea pig or rat possesses a resistance against typhus infection which llm;ts the distribution of the virus and leads to almost invariable recovery of the animals. Many of our efforts during the last 2 years have focused upon the production of increased susceptibility , in order that we might convert the milder, experimental disease into a fatal one or at least obtain an increased ...