This work studies the variability of Saccharomyces cerevisiae present during spontaneous fermentation Garnacha grapes’ musts from a “Pago” winery east Spain. The parameters used to select yeast are those related growth, fermentative behaviour, and influence on wine’s aroma polyphenolic composition. Yeast identification was performed by ITS analysis typed Hinfl mDNA restriction profile analysis....

Due to the problems encountered in routine morphological and physiological procedures that are used in yeast identification, DNA-based methods have recently been developed. In the present study, l66 yeast strains were isolated from several apple and citrus cultivars. After analysis by basic morphological methods, the ITS1 and ITS2 regions of the isolates were amplified separately, and the isola...

Yeasts are eukaryotic microorganisms including 1,500 species. They are an important model organism in modern cell biology research, and are one of the most thoroughly researched eukaryotic microorganisms. Some yeast can find potential application in the field of bioremediation. In the present investigation the yeast samples were isolated on YPD medium from natural sources like ripened fruits, m...

Yeasts are a convenient platform for many applications. They have been widely used as the expression hosts. There is a need to have a new yeast expression system to contribute the molecular cloning demands. Eight yeast isolates were screened from various environment sources and identified through ribosomal DNA (rDNA) Internal Transcribed Spacer (ITS). Full sequence of the rDNA ITS region for ea...

Introduction and objective: Esterase activity is used in evaluation and correlation of strains in fungi. Our previous study showed that Candida albicans has a kind of intracellular esterase activity in yeast extract, peptone, and glucose medium (YPG). The aim of this research was to study the qualitative and quantitative differences of this enzymatic activity among clinical isolates of this yea...

BacT/Alert FAN blood culture bottles have been shown to enhance the recovery of bacteria and yeast from blood compared with standard BacT/Alert bottles. It is well established that standard BacT/Alert blood culture bottles require no more than 5 days of incubation for the detection of routine bacteria and yeast. It is less clear, however, whether FAN bottles also routinely require 5 days of inc...

The composition of yeast microflora in artisanal "Pecorino di Filiano" cheese, a typical product of the Basilicata region of Southern Italy, was studied during ripening. The isolates were identified by restriction analysis of the 18S rDNA amplified region with the combined use of Hinf I and Cfo I enzymes. The majority of the isolates were identified as Debaryomyces hansenii, whereas two yeasts ...

Growth factor(s) present in a spent liquid medium after culture of the yeast form of Histoplasma capsulatum enhanced both yeast and mycelial growth of nine isolates tested. Hydroxamic acid extracted from the culture fluid displayed growth factor activity.

the biocontrol activity of two isolates of candida membranifaciens against grey mold of apple fruit caused by botrytis mali and their ability to induce biochemical defense responses in apple tissue were investigated. apple fruit (malus domestica) wounds were inoculated with 50 µl yeast suspension (1 × 107 cfu/ml) of c. membranifaciens followed 4 h later by 20 µl of conidial suspension of b. mal...