Homogenates of the mid gut and digestive glands of Rhynchosciara angelae were prepared in 40% sucrose, with and without 0.5% Triton-X-100. These were subjected to disc electrophoresis and zymograms for esterases and alkaline phosphatase were prepared. In both groups the effect of Triton-X-100 was to increase the electrophoretic mobility of the enzymes concerned.

The toxicities of three synthetic surfactants to the marine macroalga, Ulva lactuca, have been examined by monitoring chlorophyll a fluorescence quenching. The anionic surfactant, sodium dodecyl sulphate (SDS), exerted no measurable toxicity over the concentration range 0–10 mg L, while presence of the non-ionic surfactant, Triton X-100 (TX), elicited a small reduction in photochemical efficien...

The origin of the large $\alpha$ particle production and incomplete fusion in reactions involving weakly-bound $\alpha$+$x$ cluster nuclei still remains unresolved. While (two-step) process breakup followed by capture ``free" complementary fragment ($x$) is widely believed to be responsible, a few recent studies suggest dominant role (direct) stripping. To achieve an unambiguous experimental di...

The surface membrane glycoprotein composition of human spermatozoa has been studied by introducing radioactive label into galactosyl (Gal) and N-acetylgalactosaminyl (GalNAc) residues by using the galactose oxidase/NaB3H4 method. Triton X-100 extracts and Triton X-100-resistant cytoskeletal residues were subjected to analysis by polyacrylamide gel electrophoresis. The distribution of the radiol...

Giraldez, Roberto R., Alexander Panda, and Jay L. Zweier. Endothelial dysfunction does not require loss of endothelial nitric oxide synthase. Am J Physiol Heart Circ Physiol 278: H2020–H2027, 2000.—Whereas altered nitric oxide (NO z) formation from endothelial nitric oxide synthase (NOS) causes impaired vascular reactivity in a number of cardiovascular diseases, questions remain regarding how e...

Whereas altered nitric oxide (NO.) formation from endothelial nitric oxide synthase (NOS) causes impaired vascular reactivity in a number of cardiovascular diseases, questions remain regarding how endothelial injury results in impaired NO. formation. It is unknown if loss of NOS expression or activity is required or if other factors are involved. Detergent treatment has been used to induce endo...

Recently Brown eta/. (Science 250, 1991) showed that Triton's internal heat source could amount to 5-20% of the absorbed insolation on Triton, thus significantly affecting volatile transport and atmospheric pressure. Subsequently, Kirk and Brown (Proc. 22cd LPSC, 1991) used simple analytical models of the effect of internal heat on the distribution of volatiles on Triton's surface, confirming t...

In pigs, intracytoplasmic sperm injection (ICSI) efficiency is still poor. The inadequate decondensation of the sperm chromatin, its transformation into the male pronucleus (MPN) together with the subsequent inability to activate the oocyte, seem to be the main causes of the low ICSI efficiency. In order to improve the MPN formation we took two different approaches. On the one hand, the in vitr...

Experiments were performed to determine whether platelets contain a membrane skeleton. Platelets were labeled by a sodium periodate/sodium [3H]borohydride method and lysed with Triton X-100. Much of the filamentous actin could be sedimented at low g forces (15,600 g, 4 min), but some of the actin filaments required high-speed centrifugation for their sedimentation (100,000 g, 3 h). The latter f...

The potential relationship of an intact membrane organization for the synthesis of chondroitin and chondroitin 4-sulfate was examined after modification of a mouse mast cell microsomal system with the nonionic detergent, Triton X-100. The results indicated that Triton X-100 had no effect on the rate of polymerization but had a slight effect on the size of glycosaminoglycan chains. An "all or no...