Triglycerides of toad bladder epithelium have been labeled in vitro with either palmitate-1-(14)C or linoleate-1-(14)C, during incubation of bladders that had been cut in halves. Hydrolysis with pancreatic lipase of triglycerides labeled in this fashion revealed that palmitate-1-(14)C appeared predominantly in the 1- and 3-position, whereas half of linoleate-1-(14)C was located in the 2-positio...

Historically, triglycerides have been measured in the fasting state for 2 reasons. First, because of the marked increase in triglycerides after fat ingestion, the variability in triglyceride measurements is much smaller in the fasting state. Second, before the availability of direct assays for LDL cholesterol (LDL-C), estimation of LDL-C was performed in clinical practice almost exclusively by ...