We examined the function of the rice (Oryza sativa L.) antiporter-regulating protein OsARP by overexpressing it in tobacco (Nicotiana tabacum L.). In public databases, this protein was annotated as a putative Os02g0465900 protein of rice. The OsARP gene was introduced into tobacco under the control of the cauliflower mosaic virus 35S promoter. The transformants were selected for their ability t...

genic crops is considered a major threat to the durability of these crops (van Rie, 1991; Gould, 1994; McGaughey, Seed mixtures are recommended as a strategy to minimize or avoid 1994). Refugia in the form of susceptible (nontranshaving insects develop resistance to insect-resistant transgenic crops. genic) plants to prevent exposure of some portion of The objective of this study was to evaluat...

Tobacco (Nicotiana tabacum) was recently shown to detoxify heavy metals by exudation of metals as a metalsubstituted calcite (calcium carbonate) through leaf trichomes. In this paper, we describe the applications of the variablepressure scanning electron microscopy (VP-SEM) system to investigate tobacco trichomes exudates after heavy metal treatment. An energy-dispersive X-ray analysis (EDX) sy...

The present study highlights the molecular regulation of iron transport in soyFER1-overexpressing transgenic rice. Accumulation of iron in three different seed developmental stages, milk, dough, and mature, has been examined. The transgenic seeds of the milk stage showed significant augmentation of iron and zinc levels compared with wild-type seeds, and similar results were observed throughout ...

Repeated monitoring for escaped transgenic crop plants is sometimes necessary, especially in cases when the crop has not been approved for release into the environment. Transgenic oilseed rape (Brassica napus) was detected along roadsides in central Japan in a previous study. The goal of the current study was to monitor the distribution of transgenic oilseed rape and occurrence of hybridization...

Rice (Oryza sativa), a monocotyledonous plant that does not cold acclimate, has evolved differently from Arabidopsis (Arabidopsis thaliana), which cold acclimates. To understand the stress response of rice in comparison with that of Arabidopsis, we developed transgenic rice plants that constitutively expressed CBF3/DREB1A (CBF3) and ABF3, Arabidopsis genes that function in abscisic acid-indepen...

Due to the importance of chitinolytic enzymes for insect, nematode and fungal growth, they are receiving attention concerning their development as biopesticides or chemical defense proteins in transgenic plants and as microbial biocontrol agents. Targeting chitin associated with the extracellular matrices or cell wall by insect chitinases may be an effective approach for controlling pest insect...