R. García-Gonzáles, K. Quiroz, B. Carrasco, and P.D.S. Caligari. 2010. Plant tissue culture: Current status, opportunities and challenges. Cien. Inv. Agr. 37(3): 5-30. In the last two decades plant biotechnology applications have been widely developed and incorporated into the agricultural systems of many countries worldwide. Tissue culture tools have been a key factor to support such outcomes....

In nature, annual plants respond to abiotic stresses by activating a specific genetic program leading to early flowering and accelerated senescence. Although, in nature, this phenomenon supports survival under unfavorable environmental conditions, it may have negative agro-economic impacts on crop productivity. Overcoming this genetic programing by cytokinins (CK) has recently been shown in tra...

Functional genomics could be investigated by over-expressing or silencing a gene sequence towards a better understanding of its biological function at different organisation level (plant cell, tissue, organ or even in the whole plant) in response to various situations (plant developmental stages, environmental stress, plant-pathogen interactions). Actually, several trends are focusing on transi...

Expansins are cell wall proteins that are grouped into two main families, α-expansins and β-expansins, and they are implicated in the control of cell extension via the disruption of hydrogen bonds between cellulose and matrix glucans. TaEXPA2 is an α-expansin gene identified in wheat. Based on putative cis-regulatory elements in the TaEXPA2 promoter sequence and the expression pattern induced w...

Potato ( Solanum tuberosum L.) cultivar IPB CP1 is suitable as a raw material for the potato chip industry. plants are sensitive to various abiotic stresses such drought, aluminium and salinity, which induce reactive oxygen species (ROS). ROS very toxic plant cells. Superoxide dismutase (SOD) one of enzymes that catalyse H2O2 O2. This study aimed establish transgenic cv. containing MmCuZn‐SOD g...

The tomato plant (Solanum lycopersicum Mill.) is sensitive to cold, and low field temperatures can result in shortened growth periods and decreased crop yield. Transcription of CRT/DRE-binding factor (CBF) is regulated by INDUCER of CBF EXPRESSION1 (ICE1). CBF activates many downstream genes that confer cold tolerance on plants. ICE1 has been used in genetic engineering to improve cold-resistan...

To explore possible sources of transgenic resistance to the rhizomania-causing Beet necrotic yellow vein virus (BNYVV), Nicotiana benthamiana plants were constructed to express the harpin of Pseudomonas syringae pv. phaseolicola (HrpZ(Psph)). The HrpZ protein was expressed as an N-terminal fusion to the PR1 signal peptide (SP/HrpZ) to direct harpin accumulation to the plant apoplast. Transgene ...

Introduction Application on industrial production of biomolecules Human therapeutics from recombinant DNA technology Success story of Insulin Host used for therapeutic production Safety of the recombinant biomolecules Human monoclonal antibody Application in Agriculture Development of stress tolerant plant Development of plant having improved yield Transgenic plant as a source of bio pharmaceut...

Controversy regarding genetically modified (GM) plants and their potential impact on human health contrasts with the tacit acceptance of other plants that were also modified, but not considered as GM products (e.g., varieties raised through conventional breeding such as mutagenesis). What is beyond the phenotype of these improved plants? Should mutagenized plants be treated differently from tra...

The evaluation of transgenic plants which is usually carried out under controlled conditions in culture rooms and greenhouses can yield valuable information about the influence of introduced genes on a transgenic plant phenotype. However, an overall assessment of plant performance can only be made by testing transgenic plants in the field environment. Thus, the effects of pyramided rice cystati...