Abstract In eukaryotes three major nuclear RNA Polymerases (Pols I, II and III) transcribe the genome. Pols III many different genes. Pol I has only one target from which it synthesizes precursor for 3 of 4 ribosomal (r)RNAs accounting up to 60 percent total cellular RNA. Dedication its specific transcription factors a single gene underlines importance rRNA synthesis. Research in Regensburg aim...

Diffuse large B-cell lymphoma is the most common type of non-Hodgkin lymphoma. MicroRNAs (miRNAs) are endogenous small RNA, which can regulate gene expression at the post-transcriptional level. MiRNA profiling has shown a great potential as novel diagnostic and prognostic biomarkers. The present study was performed at the Nemazee Teaching Hospital (Shiraz, Iran) from 2011 to 2013.The aim of thi...

background hepatitis c virus (hcv) infection is one the major health concern among the infected transplant patients. considering several complications of the disease in them, pre and post-transplantation studies should be performed for monitoring of the infection, as well as, developing new treatment protocols. objectives the current study was conducted to determine the hepatitis c virus rna le...

Chalcone synthase (CHS) is a plant-specific enzyme that synthesises naringenin chalcone, an essential precursor of the flavonoid biosynthetic pathway. Naringenin and kaempferol are two flavonoids that have been demonstrated to inhibit the proliferation of HeLa cells. To study chalcone synthase gene regulation in Melastoma decemfidum, we developed a high-yield total RNA isolation method to assem...

Background: RNAi (RNA interference) is a new strategy in gene therapy and biotechnology which provides new promises in the treatment of different diseases such as cancer and viral diseases. CCND1 which is a key gene in cell cycle is amplified and over expressed in esophageal cancer. The objective of this study was production and siRNAs for CCND1, the key gene in cell cycle. Materials and Metho...

As technological platforms, approaches such as next-generation sequencing, microarray, and qRT-PCR have great promise for expanding our understanding of the breadth of molecular regulation. Newer approaches such as high-resolution RNA sequencing (RNA-Seq)(1) provides new and expansive information about tissue- or state-specific expression such as relative transcript levels, alternative splicin...

RNA levels in a cell are determined by the relative rates of RNA synthesis and decay. State-of-the-art transcriptional analyses only employ total cellular RNA. Therefore, changes in RNA levels cannot be attributed to RNA synthesis or decay, and temporal resolution is poor. Recently, it was reported that newly transcribed RNA can be biosynthetically labeled for 1-2 h using thiolated nucleosides,...