Commercial precoated thin-layer chromatography (TLC) and highperformance TLC (HPTLC) plates with spherical or irregular particles have been compared on the basis of theoretical plate number (N), resolution (R), linearity, development time, and limit of sensitivity for analysis of a multicomponent analgesic tablet in the fluorescence quenching mode and analysis of a five-component dye mixture in...

Nonmitochondrial ADP/ATP translocase is an energy parasite enzyme. Its encoding gene, tlc, is found only in Rickettsiales, Chlamydiales, and plant and alga plastids. We demonstrate the presence of tlc in Parachlamydia acanthamoebae. This gene shares more similarity with the tlc1 gene of Chlamydiaceae and the tlc of plant and alga plastids than with the tlc2 gene of Chlamydiaceae. Phylogenetic a...

The sequential extraction of Teucrium montanum L. was realized with five solvents of different polarities (70% methanol, petroleum ether, chloroform, ethyl acetate, n-butanol). The scavenging activity (SA) of obtained extracts was tested by measuring their ability to scavenge stable 2,2`-diphenyl-1-picrylhydrazyl (DPPH) free radical using electron spin resonance (ESR) spectroscopy. The results ...

Myrtle (Myrtus communis L.) leaf, stem and berry extracts were prepared to examine the total phenolics, flavonoids and condensed tannins contents. The antioxidant activities of crude extracts, ethyl acetate fraction, butanolic fraction, tannins and anthocyanins from these parts of plant were evaluated in vitro using reducing power and DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging...

Identification, especially of drugs, plays an important role in pharmaceutical analysis. Thin-layer chromatography (TLC) has been widely applied in this field, but sometimes its resolving power leaves something to be desired. Barbiturates, steroids, sulphonamides and local anaesthetics, for example, represent classes where an abundance of closely similar derivatives are available. Separations a...

THIN-LAYER CHROMATOGRAPHY (TLC) has been widely applied to the qualitative analysis of complex biologic lipid mixtures (1, 2), as well as to the quantitation of selected biochemical entities (3). Quantitation by TLC has been limited,for the most part,to the recovery of separated components from the chromatoplate, followed by an independent analysis using established analytic methods. Current te...

The aim of the study was to scientifically evaluate the antimycobacterial activity of selected indigenous medicinal plants from the Limpopo Province used for the treatment of humans with symptoms of Mycobacterium tuberculosis. The leaves of five plant species (Apodytes dimidiata, Artemisia, Combretum hereroense, Lippia javanica, and Zanthoxylum capense) were collected from the Lowveld National ...

Cytotoxic, antioxidant, tyrosinase inhibitory, antimicrobial activities of the crude ethanol extract of dry powdered roots of Asparagus racemosus (Liliaceae) were investigated. The LC(50) to brine shrimp was 2189.49 microg/ml; the EC(50) for DPPH radical scavenging was 381.91 microg/ml; the IC(50) for tyrosinase inhibition was 7.98 mg/ml. The extract was active at 5-20 mg/ml against various pat...

In the present study, olive leaf extract, harvested from four seasons, extracted with solvent (methanol and water), was treated by D101 and Sephadex LH-20, and divided into 10 fractions by TLC detection. The antioxidant activity was evaluated using DPPH, superoxide anion scavenging and reducing power assays. Tyrosinase inhibitory activity was also performed with L-DOPA as substrate. On extracti...