We thoroughly analyze the linear propagation effects that affect polarization-resolved Second Harmonic Generation imaging of thick anisotropic tissues such as collagenous tissues. We develop a theoretical model that fully accounts for birefringence and diattenuation along the excitation propagation, and polarization scrambling upon scattering of the harmonic signal. We obtain an excellent agree...

Widespread studies on tumor diagnosis has led to the development of techniques with greater sensitivity and improved specificity for detection of occult and small subclinical tumors. Tumor imaging by radiolabeling of tumor-specific antibodies (Immunoscintigraphy) or tissue specific biochemical agents (Receptor imaging) and especially physiologic imaging with positron emitters (PET), have ...

The biological and mechanical function of connective tissues is largely determined by controlled cellular alignment and therefore it seems appropriate that tissue-engineered constructs should be architecturally similar to the in vivo tissue targeted for repair or replacement. Collagen organisation dictates the tensile properties of most tissues and so monitoring the deposition of cell-secreted ...

OBJECTIVE To compare the use of conventional, real-time compound, and pulse-inversion harmonic imaging in the evaluation of breast nodules. MATERIALS AND METHODS Fifty-two breast nodules were included in this study, conducted between May and December 2000, in which conventional, real-time compound, and pulse-inversion harmonic images were obtained in the same plane. Three radiologists, each b...

Loss of myelin in the central nervous system (CNS) leads to debilitating neurological deficits. High-resolution optical imaging of myelin in the CNS of animal models is limited by a lack of in vivo myelin labeling strategies. We demonstrated that third harmonic generation (THG) microscopy-a coherent, nonlinear, dye-free imaging modality-provides micrometer resolution imaging of myelin in the mo...

The aim of this article is to review the basic principles of two-photon excitation fluorescence (2PEF) microscopy and to compare the advantages and disadvantages of 2PEF imaging to other microscopy methodologies. 2PEF imaging is a nonlinear approach that generates images of optical sections and that is particularly well suited for deep-tissue and in vivo imaging of live animals. The nonlinear e...

Multiphoton microscopy is the preferred method when in vivo deep-tissue imaging is required. This review presents the application of multiphoton microscopy in defining liver function. In particular, multiphoton microscopy is useful in imaging intracellular events, such as mitochondrial depolarization and cellular metabolism in terms of NAD(P)H changes with fluorescence lifetime imaging microsco...

Medical ultrasound contrast imaging is a powerful modality undergoing successive developments in the last decade to date. Lately, pulse inversion has been used in both ultrasound tissue harmonic and contrast imaging. However, there was a tradeoff between resolution and penetration. Chirp excitations partially solved the tradeoff, but the chirp setting parameters were not optimized. The present ...

Contrast ultrasound imaging has become a significant imaging technique for the last ten years. Physiological and pathological information are accessible thanks to microbubble injection and nonlinearity detection. To improve resolution and penetration, frequency modulated signals called chirps combined with compression filter have been utilized by radar technology. Recent studies applied the use...

Conventional histological analyses are the gold standard for study of aneurysms and vascular pathologies in pre-clinical research. Over past decade, vivo ex imaging using multiphoton microscopy have emerged as powerful tools detailed tissue that can assess morphology, extracellular matrix (ECM), cell density vascularisation. Multiphoton allows deeper penetration with minor phototoxicity. The pr...