the aim of this study was to detect the glutathione s-transferase(gst) enzyme activity of healthy / cystic liver as a diagnostic biomarker for hydatidosis. in order to compare with liver tissue, the level of the gsts enzyme activity of parasite was also determined.parasites were collected from sheep liver tissue with hydatid cysts at a local abattoir and washed with pbs buffer. collected parasi...

Thiopurine S-methyltransferase (TPMT) is an enzyme that catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine, 6-thioguanine, and azathioprine. TPMT activity exhibits an interindividual variability mainly as a result of genetic polymorphism. Patients with intermediate or deficient TPMT activity are at risk for toxicity after receiving standard doses of thiopurine drugs. The a...

We have established m-diamminedichloroplatinum(II) (cisplatin) re sistant human small cell lung cancer cell lines, H69/CDDP0.2 and H69/ CDDP, to investigate the mechanism of acquired resistance to cisplatin. H69/CDDP02 and H69/CDDP »ere6and 11-fold resistant to cisplatin compared with the H69 parental cell line. H69/CDDP was also resistant to cadmium chloride (2-fold), m-diammine(glycolato)pla...

Glutathione transferase A has been purified from rat liver. The enzyme catalyzes the conjugation of glutathione with compounds bearing an electrophilic site, especially those in which the electrophilic site is on, or cr to, an aromatic ring. The enzyme has a molecular weight of 45,000 and is composed of two similar subunits. Initial velocity, product inhibition, and binding studies indicate a b...

Stable analogs of bacterial transferase MraY substrate or product with a pyrophosphate surrogate in their structure are described. β-ketophosphonates were designed as pyrophosphate bioisosteres and were investigated as UDP-GlcNAc mimics. The developed strategy allows introduction of structural diversity at a late stage of the synthesis. The biological activity of the synthesized compounds was e...

1 The Two-Hybrid System: A Personal View Stanley Fields and Paul L. Bartel .............................................. 3 2 Growth and Maintenance of Yeast Lawrence W. Bergman ................................................................ 9 3 Media Formulations for Various Two-Hybrid Systems Michael Saghbini, Denise Hoekstra, and Jim Gautsch ........ 15 4 Yeast Two-Hybrid Vectors and Strain...

Oligophenylalanines are soluble in m-cresol, but oligophenylalanyl-tRNAs are not. This differential solubility can be used to assay oligophenylalanines released from tRNA during their synthesis by wheat germ extracts. When poly U is the message, virtually no free product appears. When poly A U (A < U) is used, a considerable amount of oligophenylalanines are released. The fraction of product re...

Background: Glutathione S-transferases (GSTs) are detoxifying enzymes that contribute to the glutathione-ascorbic acid (vitamin C) antioxidant cycle. Objective: The objective was to determine whether GST genotypes modify the association between dietary vitamin C and serum ascor-

The activity of the enzyme thiopurine methyltransferase (TPMT) is regulated by a common genetic polymorphism. One in 300 individuals lack enzyme activity and 11% are heterozygous for a variant low activity allele and have an intermediate activity. The thiopurine drugs azathioprine, mercaptopurine and thioguanine are substrates for TPMT; these drugs exhibit well documented myelosuppressive effec...